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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution (4.6 mg/L) was prepared by directly adding 9.23 mg of test substance to 2L of test medium. The mixture was placed in an ultrasonic bath for 10 minutes then stirred for about 1 day at 20 ± 2 °C. After stirring the stock solution was clear and colorless with no visible undissolved test substance. However, by request of the sponsor, the stock solution was centrifuged (approx. 20 min at about 17700 G with a Beckman CoulterTM, Avanti J-25) to assure the removal of any undissolved test substance. The lower test concentrations were prepared by dilution of this stock solution. Before the dilution was performed, the stock solution was checked for complete dissolution of the test substance.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Ecotoxicology Laboratory of Experimental Toxicology and Ecology, BASF SE, Ludwigshafen Germany.
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)
- Feeding during test: none

ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): yes
- Type and amount of food: unicellular algae (Desmodesmus subspicatus)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
2.20 – 3.20 mmol/L
Test temperature:
20 ± 2 °C.
pH:
7.8-7.9
Dissolved oxygen:
8.6-9.0 mg/L
Details on test conditions:
0, 0.1, 0.22, 0.46, 1.0, 2.2 and 4.6 mg/L (nominal)
0, 0.09, 0.19, 0.41, 0.91, 2.0 and 4.3 mg/L (mean measured)
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.19 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.35 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL = 0.18-0.71 mg/L
Details on results:
- Effect concentrations exceeding solubility of substance in test medium: no
Results with reference substance (positive control):
- Results with reference substance valid? yes
- EC50/LC50: The EC50(24h) of the reference substance potassium dichromate was 1.04 mg/L (experiment date: 09 Dec 2010, project number: 50E0063/043039). This result is within the range of 0.6 – 2.1 mg/L (ISO 6341) and indicates that the culture of Daphnia magna used in this study is responding normally to toxic stress.
Validity criteria fulfilled:
yes
Conclusions:
Acutely very toxic to aquatic invertebrates.

Description of key information

After 48 hours of exposure an EC50 of 0.35 mg/L was estimated (BASF, 2011), related to the mean measured concentration. This result indicates that the substance is acutely very toxic to aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
0.35 mg/L

Additional information

Acute toxicity to aquatic invertebrates was investigates in a static approach according to GLP and OECD guideline 202 using Daphnia magna as test organism (BASF 2011). After 48 hours of exposure an EC50 of 0.35 mg/L was estimated, related to the mean measured concentration. This result indicates that the substance is acutely very toxic to aquatic invertebrates. In a semi-static approach according to the same guideline and with the same test species a slightly higher EC50 of 2.47 mg/L was determined (Mitsubishi, 2002). The more sensitive value of 0.35 mg/L was used for GHS classification and PNEC derivation.


Two non GLP studies according to OECD 202 assessed the aquatic toxicity to Moina macrocopa (EC50 (24h) = 2.3 ppm) (Fujiwara, 1982) and Daphnia magna (EC100(24h) >100 mg/L) (Rulfi & Mueller, 1986). Due to methodological and documentary deficiencies theses studies were rate less reliable than the above-mentioned studies of Mitsubishi and BASF. Nevertheless, these information can be considered as additional information.