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Diss Factsheets

Ecotoxicological information

Additional ecotoxological information

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Administrative data

Endpoint:
additional ecotoxicological information
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Follows basic scientific principles. Not a toxicological study, but provides supporting data to suggest monocalcium phosphate is well tolerated in the diets of fish.

Data source

Reference
Reference Type:
publication
Title:
Dietary phosphorus requirement of juvenile haddock (Melanogrammus aeglefinus L.)
Author:
Roy PK & Lall SK
Year:
2003
Bibliographic source:
Aquaculture. 221: 451–468

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
A study was conducted to determine the quantitative requirement, excretion, availability and deficiency signs of phosphorus in haddock. Triplicate groups of haddock (4.2F0.01 g) were fed diets containing 0.42, 0.62, 0.82, 1.02 and 1.22% P and 19 MJ digestible energy (DE) per g of diet to satiation for 12 weeks. The basal diet, containing 0.42% P (0.08 g available P per MJ DE), was supplemented with graded levels of calcium phosphate, Ca(H2PO4)2. H2O, to formulate the five experimental diets.
GLP compliance:
not specified
Type of study / information:
Availability of phosphorus from monocalcium phosphate as a food additive for fish. Indicates a low potential for toxicity.

Test material

Constituent 1
Chemical structure
Reference substance name:
Calcium bis(dihydrogenorthophosphate)
EC Number:
231-837-1
EC Name:
Calcium bis(dihydrogenorthophosphate)
Cas Number:
10031-30-8
Molecular formula:
H6CaO9P2
IUPAC Name:
calcium diphosphate hydrate
Details on test material:
- Name of test material (as cited in study report): calcium phosphate
- Molecular formula (if other than submission substance): Ca(H2PO4)2

Results and discussion

Any other information on results incl. tables

The growth, feed conversion ratio, vertebrae and opercula ash and urinary phosphate excretion were positively correlated with dietary phosphorus levels. Vertebrae ash increased from 44.5% to 56.6 ± 0.47% and operculum ash from 31.4% to 48.2 ± 0.56% of fat free dry matter with increasing dietary phosphorus content. Phosphorus requirement was estimated by using a quadratic equation for vertebrae ash. The data suggest that a diet of 0.96% total phosphorus, or 0.72% available phosphorus or 0.34 g available phosphorus per MJ DE, is required for haddock fingerlings. Plasma and urinary phosphate excretion increased with increasing dietary phosphorus levels and ranged from 0.4– 1.5 ± 0.03 to 0.1–7.9 ± 0.2 mmol- l, respectively. Inorganic phosphorus was highly available (99 ± 1.23%). Signs of phosphorus deficiency were characterized by poor growth, loss of appetite, poor bone mineralization, deformed vertebrae and an increase in body lipid content.

Applicant's summary and conclusion

Conclusions:
It is obvious from these results that P is essential for growth, efficient feed utilization and bone mineralization of haddock. Excess P not only causes excessive excretion of this element but it also has a negative effect on bone mineralization.