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Dermal absorption

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Administrative data

Endpoint:
dermal absorption in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Percutaneous absorption of aliphatic compounds.
Author:
Iwata Y, Moriya Y, Kobayashi T
Year:
1987
Bibliographic source:
Cosmet. Toiletries 102(2): 53-68.

Materials and methods

Principles of method if other than guideline:
¹⁴C labelled test substances were applied to the dorsal skin using a plaster for a 24 hour period. Immediately following application each animal was placed in a container to measure expiratory excretion. At the end of the exposure period the treated area of skin was excised and dissolved using tissue  solubiliser. The carcass was homogenised in a blender with sodium hydroxide. An aliquot of the homogenate was then dried and combusted for determination of radioactivity. The effect of different solvents and concentration of the solvent was also investigated. The role of skin irritation in absorption of test substance was examined.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Octan-1-ol
EC Number:
203-917-6
EC Name:
Octan-1-ol
Cas Number:
111-87-5
Molecular formula:
C8H18O
IUPAC Name:
octan-1-ol
Test material form:
liquid
Radiolabelling:
yes

Test animals

Species:
mouse
Strain:
other: HR/De
Sex:
not specified

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Remarks:
also tested in three solvent vehicles: TEC, castor oil and squalane.
Duration of exposure:
24 hours
Doses:
Up to 100% concentration in three vehicles and undiluted
No. of animals per group:
3
Control animals:
no

Results and discussion

Percutaneous absorption
Key result
Dose:
100%
Parameter:
percentage
Absorption:
ca. 50 %
Remarks on result:
other: 24 hours

Any other information on results incl. tables

The percentage absorbance of dose 14C-octyl alcohol (octan-1ol) = ca 50%. At least 65% of the absorbed dose is excreted as carbon dioxide in the expired air.

When tested diluted in solvent vehicles, the degree of absorption was influenced by the concentration and the type of solvent that was used.

For n-octyl alcohol in squalene, the fraction of the dose that was absorbed varied between approximately 60% of the applied dose at 0.5% concentration to just above 50% of the applied dose at 50% concentration.

For n-octyl alcohol in castor oil and in TEC (triethyl citrate), approximately 15% of the applied dose was absorbed at concentrations 0.5% - 5%, to 20% of the applied dose at 50%.

Applicant's summary and conclusion

Conclusions:
Of a dose of undiluted 1-14C-octyl alcohol applied to the skin of nude mice for 24 hours under occlusive conditions, 50 % was absorbed.
Executive summary:

Of a dose of undiluted 1-14C-octyl alcohol applied to the skin of nude mice for 24 hours, 50% was absorbed.