Calcium diformate

Neurotoxicity

Administrative data

Endpoint:

neurotoxicity

Remarks:

other: in vitro

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment. Sodium formate caused adverse effects only at high concentrations. Relevance of the in-vivo situation is unclear.

Data source

Reference

Reference Type:

publication

Title:

Histopathologic approaches to chemical toxicity using primary cultures of dissociated neural cells grown in chamber slides.

Author:

Bolon B, Dorman DC, Bonnefol MS, Randall HW, and Morgan KT

Year:

1993

Bibliographic source:

Toxicologic Pathology 21, 465-479.

Materials and methods

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Sodium formate

EC Number:

205-488-0

EC Name:

Sodium formate

Cas Number:

141-53-7

Molecular formula:

CHO2Na

IUPAC Name:

sodium formate

Details on test material:

Sodium formate.

Test animals

Species:

mouse

Strain:

CD-1

Results and discussion

Any other information on results incl. tables

Neurotoxic effects seen with sodium formate after 8 h of  incubation:

1) Toxicity in developing cultures: 
Sodium formate: toxic  at </= 40 mM. 
Toxicity of model toxicants:
Cytosine arabinoside: gliotoxic at 0.01 mM.

2) Mature cultures
Sodium formate:  neuronotoxic at </= 60 mM. 
                 cytotoxic  at >/= 120 mM.

Toxicity of model toxicants:
Glutamate neuronotoxic  at </= 5.0 mM; 
Methyl iodide neuronotoxic  at </= 2.50 mM 
               cytotoxic  at >/= 5.0 mM.

Sodium formate caused lesions mainly in large, polygonal neurons.

Applicant's summary and conclusion

Conclusions:

Neuronotoxicity in vitro seen at >/= 40 mM sodium formate