Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-185-2 | CAS number: 79-20-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Methyl acetate
- EC Number:
- 201-185-2
- EC Name:
- Methyl acetate
- Cas Number:
- 79-20-9
- Molecular formula:
- C3H6O2
- IUPAC Name:
- methyl acetate
- Details on test material:
- Molecular formula: C3H6O2
Appearance: Colorless liquid
Melting Point: -98 C
Boiling Point: 57 C @ 1013 mbar
Molecular Weight: 74.1
Specific Gravity: 0.932 g/cm at 20C
Vapor Pressure: 217 mbar @ 20 C
pH - Value in water: Neutral
Storage Conditions: dark @ 22 C
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100. Escherichia coli WP2uvrA
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 from rat liver homogenate
- Test concentrations with justification for top dose:
- 0, 4, 20, 100, 500, 2500 and 5000 ug/plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- N-ethyl-N-nitro-N-nitrosoguanidine
- benzo(a)pyrene
- other: 2-Aminoanthracene
- Rationale for test conditions:
- range finding test
- Evaluation criteria:
- statistically significant dose dependent increase
Results and discussion
Test results
- Key result
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100 and E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Remarks:
- non-toxic up to 5000 ug/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
TA100 |
||||
DOSE |
Without Activation |
With Activation |
||
ug/Plate |
Mean Value Exp 1 |
Mean Value Exp 2 |
Mean Value Exp 1 |
Mean Value Exp 2 |
0 |
168 |
129 |
168 |
148 |
4 |
178 |
137 |
178 |
136 |
20 |
170 |
133 |
170 |
137 |
100 |
169 |
140 |
169 |
146 |
500 |
185 |
139 |
185 |
135 |
2500 |
154 |
138 |
154 |
133 |
10000 |
157 |
148 |
157 |
139 |
+ ctrl |
425 |
374 |
546 |
374 |
TA1535 |
||||
DOSE |
Without Activation |
With Activation |
||
ug/Plate |
Mean Value Exp 1 |
Mean Value Exp 2 |
Mean Value Exp 1 |
Mean Value Exp 2 |
0 |
17 |
10 |
15 |
11 |
4 |
16 |
10 |
14 |
14 |
20 |
17 |
9 |
16 |
12 |
100 |
15 |
11 |
12 |
10 |
500 |
12 |
10 |
14 |
11 |
2500 |
14 |
9 |
15 |
13 |
10000 |
18 |
7 |
12 |
8 |
+ ctrl |
350 |
297 |
114 |
102 |
TA1538 |
||||
DOSE |
Without Activation |
With Activation |
||
ug/Plate |
Mean Value Exp 1 |
Mean Value Exp 2 |
Mean Value Exp 1 |
Mean Value Exp 2 |
0 |
16 |
10 |
19 |
13 |
4 |
16 |
10 |
16 |
15 |
20 |
16 |
11 |
19 |
14 |
100 |
19 |
11 |
19 |
16 |
500 |
14 |
11 |
21 |
12 |
2500 |
17 |
9 |
19 |
13 |
10000 |
14 |
9 |
19 |
15 |
+ ctrl |
439 |
376 |
491 |
348 |
TA98 |
||||
DOSE |
Without Activation |
With Activation |
||
ug/Plate |
Mean Value Exp 1 |
Mean Value Exp 2 |
Mean Value Exp 1 |
Mean Value Exp 2 |
0 |
24 |
22 |
30 |
28 |
4 |
22 |
24 |
32 |
28 |
20 |
23 |
21 |
19 |
30 |
100 |
25 |
24 |
32 |
29 |
500 |
23 |
24 |
32 |
31 |
2500 |
22 |
24 |
30 |
28 |
10000 |
24 |
24 |
31 |
28 |
+ ctrl |
342 |
402 |
471 |
487 |
TA1537 |
|||||||||
DOSE |
Without Activation |
With Activation |
|||||||
ug/Plate |
Mean Value Exp 1 |
Mean Value Exp 2 |
Mean Value Exp 1 |
Mean Value Exp 2 |
|||||
0 |
11 |
9 |
11 |
12 |
|||||
4 |
9 |
8 |
10 |
10 |
|||||
20 |
11 |
8 |
10 |
9 |
|||||
100 |
11 |
9 |
10 |
10 |
|||||
500 |
9 |
8 |
9 |
9 |
|||||
2500 |
11 |
11 |
11 |
9 |
|||||
10000 |
14 |
7 |
10 |
8 |
|||||
+ ctrl |
98 |
126 |
98 |
106 |
WP2uvrA |
||||
DOSE |
Without Activation |
With Activation |
||
ug/Plate |
Mean Value Exp 1 |
Mean Value Exp 2 |
Mean Value Exp 1 |
Mean Value Exp 2 |
0 |
72 |
55 |
74 |
57 |
4 |
75 |
54 |
75 |
53 |
20 |
74 |
55 |
77 |
56 |
100 |
69 |
61 |
78 |
55 |
500 |
75 |
55 |
75 |
52 |
2500 |
75 |
57 |
79 |
52 |
10000 |
76 |
55 |
75 |
55 |
+ ctrl |
280 |
509 |
216 |
446 |
Applicant's summary and conclusion
- Conclusions:
- Not Mutagenic with or without metabolic activation
- Executive summary:
Methyl acetate was tested for mutagenicity with the strains TA 100, TA 1535, TA 1537, TA 1538, TA 98 of Salmonella typhimurium and Escherichia coli WP2uvrA.
The mutagenicity studies were conducted in the absence adn in the presence of a metabolizing system derived from rat liver homogenate. A dose range of 6 different doses from 4 micrograms/plate to 5000 micrograms/plate was used.
Control plates without mutagen showed that the number of spontaneous revertant colonies was similar to that described in the literature. All the positive control compounds gave the expected increase in the number of revertant colonies.
Toxicity: The test compound proved to be not toxic to the bacterial strains at 5000 micrograms/plate. 5000 micrograms/plate was chosen as the top dose level for the mutagenicity study.
Mutagenicity: In the absence of the metabolic activation system the test compound did not show a dose dependent increase in the number of revertants in any of the bacterial strains. Also in the presence of a metabolic activation system, treatment of the cells with Methyl acetate did not result in relevant increases in the number of revertant colonies.
Summarizing, it can be stated that Methyl acetate is not mutagenic in these bacterial test systems either with or without exogenous metabolic activation at the dose levels investigated.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
