Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 234-364-9 | CAS number: 11120-25-5
Toxicological information
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998-02-24 to 1999-06-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Well documented, scientifically sound study that was conducted in accordance to GLP and OECD guideline 402 with no deviation to the protocol.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Ammonium wolframate
- EC Number:
- 234-364-9
- EC Name:
- Ammonium wolframate
- Cas Number:
- 11120-25-5
- Molecular formula:
- (NH4)10H2W12O42*4H2O
- IUPAC Name:
- Ammonium (para)tungstate
- Reference substance name:
- Ammonium paratungstate
- IUPAC Name:
- Ammonium paratungstate
- Details on test material:
- - Name of test material (as cited in study report): Ammonium Paratungstate (CAS# as cited in study: 12028-06-7)
- Physical state: White powder
- Analytical purity: >99.9%
- Storage condition of test material: Room temperature
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan UK Ltd, Bicester, Oxon, England
- Age at study initiation: 8 to 11 weeks
- Weight at study initiation: 214 to 245 g
- Housing: Individually in metal cages with wire mesh floors.
- Diet (eg ad libitum): Special Diet ServicesRM1(E) SQC expanded pellet- ad libitum
- Water (eg ad libitum): ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 to 22.5
- Humidity (%): 29 to 52%
- Air changes (per hr): 10 to 15
- Photoperiod (hrs dark / hrs light): 12/12 artificial light (0700 - 1900)
IN-LIFE DATES: From: February 24, 1998 To:March 10, 1998
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- other: 1% w/v aqueous methylcellulose
- Details on dermal exposure:
- TEST SITE
- Area of exposure: Dorso-lumbar region
- % coverage: 10 %
- Type of wrap if used: The treatment area (approximately 50 mm x 50 mm) was covered with porous gauze with a waterproof dressing encircled firmly around the trunk of the animal.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Washed with warm water (30 to 40 °C)
- Time after start of exposure: 24 hours
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg bodyweight; maximum practical concentration of 200% w/v in 1% aqueous methylcellulose and administered at a dose volume of 1 mL/kg bodyweight.
- Constant volume or concentration used: Yes
VEHICLE
- Concentration (if solution): 1 % w/v aqueous methylcellulose - Duration of exposure:
- 24 hours
- Doses:
- Single dose of 2000 mg/kg bodyweight
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: Yes, all animals were killed on Day 15
- Mortality: Cages of rats were checked at least twice daily for any mortalities.
- Clinical signs: Animals were observed soon after dosing and at frequent intervals for the remainder of Day 1. On subsequent days animals were observed once in the morning and again at the end of the experimental day (with the exception of Day 15 -morning only). The nature of the clinical signs and time were recorded at each observation.
- Dermal responses: Local dermal irritation at the treatment site was assessed daily using the numerical scoring system presented below.
- Bodyweight: The bodyweight of each rat was recorded on Days 1 (prior to dosing), 8 and 15. Individual weekly bodyweight changes and group mean bodyweights were calculated.
- Macroscopic pathology: All animals were subjected to a macroscopic examination which consisted of opening the abdominal and thoracic cavities. The macroscopic appearance of all tissues was recorded and macroscopic abnormalities were preserved. - Statistics:
- no data
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Mortality:
- There were no deaths.
The acute lethal dose to rats of Ammonium paratungstate was demonstrated to be greater than 2000 mg/kg bodyweight. - Clinical signs:
- other: There was no evidence of a systemic response in any animal throughout the study period.
- Gross pathology:
- No macroscopic abnormalities were observed for animals killed at study termination on Day 15.
- Other findings:
- No dermal irritation was seen in any animal during the study.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The acute lethal dermal dose to rats of ammonium paratungstate was demonstrated to be greater than 2000 mg/kg bodyweight.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
