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EC number: 271-030-1 | CAS number: 68514-28-3
Exposition: 4 hours
1st Test (4 hours)
2nd Test (26 hours)
% AB.B. Frequency of aberrant cells
A- Without activation
A+ With activation
* High toxicity
Ko Negative control
Ko A Negative control with activation
CP A Cyclophosphamide
The clastogenicity potential of the test substance was determined using In Vitro Mammalian Chromosome Aberration Test.
The study was performed acocording to the OECD Guideline for the Testing of Chemicals No. 473, 1977.
The test was carried out in human peripheral blood lymphocytes with and without metabolic activation system in two separate assays.
A range of six concentrations of test substance in water solution was used in the preliminary testing with and without metabolic activation: 0.5, 1, 2, 2.5, 3 and 5 mg/mL of culture. The doses 2.0, 2.5 mg/mL partially inhibited cell activation, doses above 3 mg/mL of culture were evaluated as highly toxic.
Three concentrations - 0.5, 1.0 and 2.5 mg/mL of test substance were used in the main test. At predetermined intervals (4 and 26 hours) after exposure of cell cultures to the test substance, cells were arrested at metaphase with colchicine, harvested and slides stained. Metaphase cells were analysed microscopically for the presence of chromosome aberrations. A total of 200 well-spread metaphases were examined per concentration on coded slides. Concurrent positive (Cyclophosphamide, Thiotepa) and negative (water) controls were included in each experiment.
Under the test conditions the test substance does not induce increase of structural chromosome aberrations in cultured human peripheral blood lymphocytes.
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