Registration Dossier
Registration Dossier
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EC number: 200-848-3 | CAS number: 75-20-7
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Non-GLP, non-guideline animal experimental study, published in peer reviewed literature, limitations in reporting but otherwise adequate for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Self-catalysed inactivation of hepatic cytochrome P-450 by ethynyl substrates
- Author:
- Ortiz de Montellano, P.R., and Kunze, K.L.
- Year:
- 1 980
- Bibliographic source:
- J. Biol. Chem., 255, 5578-5585
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Acetylene gas was administered by placing phenobarbital-pretreated rats for 4 h in a chamber through which a stream of air containing 10 to 15% (v/v) (equivalent to 107,000 - 160,500 mg/m3). acetylene was passed at an approximate rate of 200 to 300 ml/min. The interaction of acetylene with hepatic P-450 was investigated in-vitro and in-vivo.
- GLP compliance:
- not specified
- Test type:
- other: in-vivo and in-vitro
- Limit test:
- no
Test material
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
Administration / exposure
- Route of administration:
- inhalation: gas
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- INHALATION EXPOSURE:
- Animals were pre-treated for 4 days with 80 mg/kg/day sodium phenobarbital in aqueous solution by intraperitoneal injection
- Pre-treated rats were exposed in a chamber through which was passed the test atmosphere at a rate of 200-300 mL/min - Duration of exposure:
- 4 h
- Concentrations:
- 10-15% v/v acetylene in air (equivalent to 107,000 - 160,500 mg/m3)
- No. of animals per sex per dose:
- no data
Results and discussion
Effect levels
- Key result
- Sex:
- male
- Dose descriptor:
- LC0
- Effect level:
- 160 500 mg/m³ air (nominal)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- no mortality
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Acute inhalation exposure of male Sprague Dawley rats to 10-15% v/v acetylene (equivalent to 107,000 - 160,500 mg/m3) for 4 hours did not result in mortality.
- Executive summary:
In an acute inhalation toxicity study, groups of male Sprague Dawley rats were exposed by inhalation route to acetylene in air for 4 hours to whole body at concentrations of 10-15% (v/v) acetylene in air (equivalent to 107,000 - 160,500 mg/m3).
There was no mortality observed after 4 hours exposure.
Based on these results, GHS criteria are not met and no classification according to Regulation (EC) No. 1272/2008 is warranted.
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