Calcium acetylide

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Non-GLP, non-guideline animal experimental study, published in peer reviewed literature, limitations in reporting but otherwise adequate for assessment

Data source

Reference

Reference Type:

publication

Title:

Self-catalysed inactivation of hepatic cytochrome P-450 by ethynyl substrates

Author:

Ortiz de Montellano, P.R., and Kunze, K.L.

Year:

1980

Bibliographic source:

J. Biol. Chem., 255, 5578-5585

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Acetylene gas was administered by placing phenobarbital-pretreated rats for 4 h in a chamber through which a stream of air containing 10 to 15% (v/v) (equivalent to 107,000 - 160,500 mg/m3). acetylene was passed at an approximate rate of 200 to 300 ml/min. The interaction of acetylene with hepatic P-450 was investigated in-vitro and in-vivo.

GLP compliance:

not specified

Test type:

other: in-vivo and in-vitro

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Acetylene

EC Number:

200-816-9

EC Name:

Acetylene

Cas Number:

74-86-2

Molecular formula:

C2H2

IUPAC Name:

acetylene

Constituent 2

Reference substance name:

74-86-5

IUPAC Name:

74-86-5

Details on test material:

- Acetylene obtained from Matheson Chemical Co.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Administration / exposure

Route of administration:

inhalation: gas

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

INHALATION EXPOSURE:
- Animals were pre-treated for 4 days with 80 mg/kg/day sodium phenobarbital in aqueous solution by intraperitoneal injection
- Pre-treated rats were exposed in a chamber through which was passed the test atmosphere at a rate of 200-300 mL/min

Duration of exposure:

4 h

Concentrations:

10-15% v/v acetylene in air (equivalent to 107,000 - 160,500 mg/m3)

No. of animals per sex per dose:

no data

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LC0

Effect level:

160 500 mg/m³ air (nominal)

Based on:

test mat.

Exp. duration:

4 h

Mortality:

no mortality

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Acute inhalation exposure of male Sprague Dawley rats to 10-15% v/v acetylene (equivalent to 107,000 - 160,500 mg/m3) for 4 hours did not result in mortality.

Executive summary:

In an acute inhalation toxicity study, groups of male Sprague Dawley rats were exposed by inhalation route to acetylene in air for 4 hours to whole body at concentrations of 10-15% (v/v) acetylene in air (equivalent to 107,000 - 160,500 mg/m³).

There was no mortality observed after 4 hours exposure.

Based on these results, GHS criteria are not met and no classification according to Regulation (EC) No. 1272/2008 is warranted.