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EC number: 200-848-3 | CAS number: 75-20-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Only very low concentrations were tested due to technical difficulties. Only 3 strains tested. No cytotoxicty screening performed.
- Principles of method if other than guideline:
- The study's main objective was to develop a technique for testing appropriately air vapors in the Ames test. Toxicity and mutagenicity of styrene oxide were evaluated on log- and stationary-phase Salmonella TA100 cells, with the preincubation and plate incorporation method. As part of this research 12 different vapor-phase substances, including acetylene, were examined in this adapted Ames test.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Acetylene
- EC Number:
- 200-816-9
- EC Name:
- Acetylene
- Cas Number:
- 74-86-2
- Molecular formula:
- C2H2
- IUPAC Name:
- acetylene
- Test material form:
- other: vapour
- Details on test material:
- Gas acetylene purchased from Matheson Scientific Company
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA97, TA98, and TA100
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver S9 derived from Aroclor 1254 induced rats and hamsters
- Test concentrations with justification for top dose:
- 0.30, 1.00, 3.00, 10.00, 31.00 μg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: acetone
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation with log-phase cells in full, not shaken vials
No cytotoxicity testing performed due to difficulties in preparing the right sample with acetylene.
DURATION
- Preincubation period: 48 h
- Exposure duration: 10 min
NUMBER OF REPLICATIONS: three
DETERMINATION OF CYTOTOXICITY
- Method: viability index - Evaluation criteria:
- positive response: at least a two-fold increase in spontaneous revertants over background at two increasing dose levels
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Remarks:
- Due to technical difficulties: it is difficult to get high test concentrations of the gas acetylene in liquid suspension.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Remarks:
- Due to technical difficulties: it is difficult to get high test concentrations of the gas acetylene in liquid suspension.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Remarks:
- Due to technical difficulties: it is difficult to get high test concentrations of the gas acetylene in liquid suspension.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- not determined
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- not determined
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- not examined
- True negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Additional information on results:
- Due to technical difficulties (getting high test concentrations of the gas acetylene in liquid suspension), acetylene was not tested for its toxic potential. The solubility of the gas in the test solvent limited the highest test concentration applied. The highest concentration applied was only 31 μg/plate. The raw data are presented in the attached pdf document.
- Remarks on result:
- other: all strains/cell types tested
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results
negative
Acetylene did not induce any mutagenic effects on S.typhymurium strains TA97, TA98 and TA100, when tested under the conditions of this test, both with and without metabolic activation. However, due to technical difficulties for the generation of higher concentrations, concentration levels were only up to 31 μg/plate. - Executive summary:
The main objective of this study was to validate an appropriate method in order to test vapor-phase compounds in the Ames mutagenicity test. Several parameters were investigated and 12 vapor phase substances, including acetylene, were tested in the Ames test in order to validate the protocol. S. typhimurium strains TA97, TA98 and TA100 were exposed to acetylene, in acetoneat concentrations of 0.30, 1.00 , 3.00, 10.00 and 31.00 μg/plate in the presence and absence of mammalian metabolic activation (Aroclor-induced hamster and rat liver S9) in the pre-incubation method. The response was considered positive when at least a two-fold increase in spontaneous revertants over background at two increasing dose levels was detected.Acetylene could not be tested up to cytotoxic concentrations since no cytotoxicity screening was performed, due to difficulties of preparing the sample. Due to the difficulty of getting high test concentrations on the gas acetylene in liquid suspension, the highest concentration tested was only 31.00 μg/plate. The results revealed no mutagenicity response.The positive controls induced the appropriate responses in the corresponding strains.The authors of the study report suggested that additional experiments shall be peformed with some of the vapors, including acetylene, in order to be possible to test higher concentration levels.
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