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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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biodegradation in water: simulation testing on ultimate degradation in surface water
Data waiving:
study scientifically not necessary / other information available
Justification for data waiving:
the study does not need to be conducted because the substance is readily biodegradable
Reason / purpose for cross-reference:
data waiving: supporting information
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23 Feb 2005 - 24 Mar 2005 (experimental)
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study conducted in compliance with GLP regulations
equivalent or similar to guideline
OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
Principles of method if other than guideline:
ISO 14593 Water quality- Evaluation in an aqueous medium of the ultimate aerobic biodegradability of organic compounds - Method by analysis of released inorganic carbon in sealed vessels (1999).
GLP compliance:
yes (incl. QA statement)
Oxygen conditions:
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): from laboratory waste water treatment plant , fed with municipal sewage.
- Pretreatment: 24 h aeration
- Concentration of sludge: 4 mg/l dry substance
Duration of test (contact time):
28 d
Initial conc.:
20 mg/L
Based on:
Initial conc.:
35 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
inorg. C analysis
Details on study design:
- Composition of medium: 90 ml/vessel mineral medium, 0.5 ml/vessel inoculum and 3.7 µl/vessel test substance filled up to a total volume of 100 ml
- Test temperature: 22 +/- 2 °C
- pH: 7.,4 +/-0.2
- pH adjusted: yes (1. mol sulfuric acid)

- Number of culture flasks/concentration: 1
- Test performed in closed vessels due to significant volatility of test substance: yes

- Sampling frequency: on days 3, 7, 10, 14, 17, 21 and 28

- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance:
% degradation (inorg. C analysis)
90 - 100
Sampling time:
28 d
Remarks on result:
other: TIC/ThIC
Details on results:
Duration of adaptation phase (days): 1
Duration of degradation phase (days): 20
Degradation degree of the test substance at the end of exposure (% TIC/ThIC): 90-100
Degradation of the test substance at the end of the 10-day window (% TIC/ThIC): 80-90
Degradation degree in the inhibition control 14 days (% TIC/ThIC) 80-90
Physical-chemical (abiotic) elimination of the test substance at the end of exposure (% TIC/ThIC): <10

The test substance is readily biodegradable (according to OECD criteria).

Kinetic of test substance (in %):
= 54 after 3 day(s)
= 85 after 7 day(s)
= 86 after 10 day(s)
= 91 after 14 day(s)
= 97 after 21 day(s)
Kinetic of control substance (in %):
= 63 after 3 day(s)
= 99 after 14 day(s)
Results with reference substance:
Degradation degree of the reference substance after 14 days (% TIC/ThIC): 90-100
Validity criteria fulfilled:
Interpretation of results:
readily biodegradable

Data source

Materials and methods

Results and discussion

Applicant's summary and conclusion