methyl acrylate; methyl propenoate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study acceptable for assessment (restrictions: post-exposure observation time only 7 days; only one sex tested; high application volume)

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1958

Report date:

1958

Reference 2

Reference Type:

secondary source

Title:

Report on toxicological studies with different acrylic acid esters (in German).

Author:

BASF AG

Year:

1958

Bibliographic source:

cited in: MAK Begruendung Methylacrylat (1985) (in German)

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

BASF Test. In principle, the methods described in OECD Guideline 401 were used.
Several groups of 5 and 10 rats per dose respectively were treated by gavage with preparations of the test substance in 10 % traganth. Group-wise documentation of clinical signs was performed over the 7-day study period. Body weight was determined before the start of the study only, as it was needed for determination of dose. The clinical signs and findings were reported in summary form.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl acrylate

EC Number:

202-500-6

EC Name:

Methyl acrylate

Cas Number:

96-33-3

Molecular formula:

C4H6O2

IUPAC Name:

methyl prop-2-enoate

Details on test material:

- Name of test material (as cited in study report): Acrylmethylsaeureester
- Purity: no data

Test animals

Species:

rat

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 150 - 208 g
no further data

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: 0.5 % aqueous Traganth emulsion

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 2 %


MAXIMUM DOSE VOLUME APPLIED: 63 mL/kg b.w.

Doses:

196, 303, 481, 762 and 1210 mg/kg bw (calculated with a density of 0.96 g/mL; original data were 204, 316, 501, 794 and 1260 mL/kg)

No. of animals per sex per dose:

5 males per dose

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 d, daily observation and necropsy of all survivors

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 768 mg/kg bw

Remarks on result:

other: original value: 800 µL (calculated with a density of 0.96 g/mL

Mortality:

No animals died in the 3 lowest dose groups, but 2/5 and 5/5 died in the 762 and the 1210 mg/kg groups, respectively. Deaths occurred within 24 hours in the highest dose group, and within 48 hours in the 762 mg/kg group.

Clinical signs:

other: Clinical signs were staggering and prone position in the highest dose group.

Gross pathology:

Lesions of the gastric mucosa.

Any other information on results incl. tables

Cumulative Mortality

	Mortality within
Dosis (mg/kg bw)	1 h	24 h	48 h	7 d
1210	0/5	5/5	5/5	5/5
762	0/5	1/5	2/5	2/5
481	0/5	0/5	0/5	0/5
303	0/5	0/5	0/5	0/5
196	0/5	0/5	0/5	0/5

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU