Acetic anhydride

Biodegradation in soil

Administrative data

Endpoint:

biodegradation in soil

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non-standard, acceptable with restrictions

Data source

Reference

Reference Type:

publication

Title:

Turnover of 14C-labelled oat residues and smaller molecular organic compounds in two soils under different levels of mineral nutrition.

Author:

Nowak, G. , Nowak, J.

Year:

1990

Bibliographic source:

Plant and soil 122: 67-77

Materials and methods

Principles of method if other than guideline:

Non-standard. Degradation was invetigated in two acidic soils collected in northeastern Poland: a light loamy sand and a medium clay loam. 5 replicates each were tested. 34.42mg acetate were added to 100g soils together with 9 times the amount cellulose. The soil was incubated for 120days at 20°C and at 60% MWHC (33kPa).

GLP compliance:

no

Test type:

laboratory

Test material

Test material information

Constituent 1

Reference substance name:

Acetic acid

EC Number:

200-580-7

EC Name:

Acetic acid

Cas Number:

64-19-7

Molecular formula:

C2H4O2

IUPAC Name:

acetic acid

Details on test material:

[14C]-acetate. Uniformly labelled from Center for Nuclear research, Dubna, USSR.

Study design

Oxygen conditions:

aerobic

Soil classification:

not specified

Soil properties

Soil properties 1

Soil no.:

#1

Soil type:

loamy sand

% Org. C:

1.02

pH:

5.6

CEC:

1.8 meq/100 g soil d.w.

Soil properties 2

Soil no.:

#2

Soil type:

clay loam

% Org. C:

0.83

pH:

4.7

CEC:

2.4 meq/100 g soil d.w.

Details on soil characteristics:

Both soils were medium-rich in available potassium and magnesium. Low availability of phosphorous in the sandy soil, medium in the clay soil.

Duration of test (contact time)

Duration of test (contact time) 1

Soil No.:

#1

Duration:

120 d

Duration of test (contact time) 2

Soil No.:

#2

Duration:

120 d

Parameter followed for biodegradation estimation:

CO2 evolution

Experimental conditions

Experimental conditions 1

Soil No.:

#1

Temp.:

20C

Humidity:

60%

Experimental conditions 2

Soil No.:

#2

Temp.:

20C

Humidity:

60%

Details on experimental conditions:

34.42 mg of acetate were added to 100g of each soil, with 9 times the amount of cellulose.

Results and discussion

Half-life / dissipation time of parent compound

Soil No.:

#1

DT50:

2 d

Type:

not specified

Temp.:

20 °C

Remarks on result:

other:

Remarks:

Temperature not stated

Transformation products:

not specified

Details on results:

The radioactivity was found as CO2at approximately 2-6% on day 2, increasing to approx 20% on day 120. The rest of the AR was incorporated in humic acids, fulvic acids and humine. The residues found in the organic acids was approximately constant. The mineralisation amounts approximately 20% for both soils after 120 days (III) and the bound residues were arisen for approximately 80% (class IV).

Any other information on results incl. tables

Recovery rates for¹⁴C were 84 -99% in the light loamy sand and 74 -98% in the medium clay soil.

Mineralisation amounts were approximately 20% after 120 days and the non-extractable residues was approximately 80% after 120 days.

Applicant's summary and conclusion

Conclusions:

The primary degradation of acetate is fast, and complete incorporation into organic substances occured within 2 days. The main part was incorporated into humic or fulvic acids. In soil, acetic acid is metabolised fast within 2 days.

Executive summary:

This study is non-GLP and no guideline. It lacks detail but provides an indication that acetic acid is degraded rapidly in soil.