Sebacic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

- The test substance was added direct into the study beaker.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Supplier: Yara Tropical Fish s.r.l., Ivrea
- Body weight and length (average values) at the beginning of the study: about 0.47 g and about 3 cm
- The fish are exposed to artificial lighting with a light period of 16 hours daily
- During the acclimation the animals were fed daily with Sera sikus food. During the test the fish were fasted.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

about 228 mg of CaCO3

Test temperature:

20-22 °C

pH:

7.1-7.9

Dissolved oxygen:

7.0-8.9 mg/L

Nominal and measured concentrations:

- Nominal test concentration 100 mg/L

Details on test conditions:

- Test water was reconstituted water with Ca:Mg rate of 4:1 and a Na:K rate of 10:1
- Test vessels: glass beakers containing 5 liters
- A group of 7 animals were tested
- Treatment solution were changed every 24 hours
- Loading: maximum loading was lower than 1.0 g fish / L (about 0.46 g/L)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- No animals in the control or treated group died.
- No animals of the control or treated group showed visible signs of abnormalities.
- The values of dissolved oxygen, pH and temperature always remained within the acceptance limits.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Qualifier:

according to guideline

Guideline:

other: Paris Commission Guideline (PRACOM 2006): Protocols on Methods for the Testing of Chemicals Used in the Offshore Oil Industry. Part B: Protocol for a Fish Acute Toxicity Test.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was added directly into the aquaria, without using carrier or solvent. The test solutions were stirred for 1 minute. The test substance was stated to be insoluble in water, the amount of test substance added to the water in this way is referred to as nominal concentration, and should not be confused with the actual, but unknown, concentration in the water phase. The solutions were prepared by the same procedure for renewal of the test solutions after 48 hours.

Test organisms (species):

other: Scophthalmus maximus

Details on test organisms:

- The Scophthalmus maximus used in the test were hatched 28.07.09 in Denmark, and were transported by car and airplane to Eurofins on 13.11.09.
- At Eurofins, they were held at a temperature of 16.0°C.
- In the test period the fish was 54 - 59 mm long, and had an average weight of 3.6 g.

Test type:

semi-static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

14.9-15.4 °C

pH:

Initial 8.2

Dissolved oxygen:

97-99 % saturation

Salinity:

34.4 per mill

Nominal and measured concentrations:

Nominal test concentration 18 mg/L

Details on test conditions:

TEST SYSTEM
- Two aquaria contained the reference substance 3,5-Dichlorophenol at 0.5 and 1.5 mg/L, and one aquarium contained the blank (10 litres of seawater with no added chemicals).
- Each aquarium contained 10 litres of test solutions and 7 test organisms.
- The aquaria were oxygenated during the test.
- The test is semi-static, in that all the solutions (including the blank) were renewed after 48 hours.
- The test was run under 12 hours light / 12 hours dark photoperiod

TEST MEDIUM / WATER PARAMETERS
- Natural seawater from an unpolluted site at about 80 m depths in Byfjord was collected from a tap.
- The seawater was oxygenated and temperature adjusted, before use as dilution water, control and for acclimation of the fish.
- The aquaria were filled with 5 litre of seawater before the test substance was added.

EFFECT PARAMETERS MEASURED
- Lethality (number of dead organisms) was registered daily by visual observation.
- Measurements of oxygen was done daily, temperature was measured at start, while pH and salinity were measured at start, day two and day four.

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenol

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was observed.

Results with reference substance (positive control):

The percentage mortality in the blank: was less than 10%, and the 96 hours test result with the 3,5dichlorphenol tumed out to be as expected.

Description of key information

With high probability sebacic acid is acutely not harmful to fish.

Additional information

According to Paris Commission Guideline a limit test with the marine fish species Scophthalmus maximus was conducted [BASF SE, 2009]. The nominal test concentration of 18 mg/L was based on former results derived from the most sensitive test species of the other taxonomic groups. (48 h LC50 value of the marine copepod Acartia tonsa, cf. chapter 6.1.3). In the limit test with the marine fish no significant mortality occurred.

Furthermore, the toxicity of sebacic acid was tested on freshwater fish Brachydanio rerio in a semi-static limit test according to OECD 203 [Ausimont S.p.A., 1999]. The result with freshwater fish confirms the result from the marine species, since no mortality observed at the test concentration of 100 mg/L sebacic acid.