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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

The test substances, Fluidized Bed Combustion (FBC) Fly Ash and Fluidized Bed Combustion (FBC) Bottom  Ash, were tested for growth inhibition on algae..
The test was performed according to method C.3 - Algal Inhibition Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
Since the test substances are poorly soluble in dilution water at concentrations needed for the test, a stock saturated solution (nominal concentration 100 mg.L-1) of the test substance in dilution water was prepared for preliminary and limit test.
The studies were performed without the analytical determination of the test substances concentrations. The nominal concentrations of the test substance were used for all evaluations and results. Only concentrations of metals in stock saturated solution were analysed in the limit test.

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L
EC10 or NOEC for freshwater algae:
63 mg/L

Additional information

Fluidized Bed Combustion (FBC) Fly Ash: The highest inhibition of cell growth in limit test was 36.1 %. The highest inhibition of growth rate in limit test was 9.1 %. Therefore exact values of EbC50 and ErC50 could not be calculated and the values of EC are given in the form of a range.

 

Fluidized Bed Combustion (FBC) Bottom Ash: The highest inhibition of growth rate in full test was 20.8 %. Therefore exact value of ErC50 could not be calculated and the value of ErC50 is given in the form of a range.

The value of EbC50 was calculated by means of software “Toxicity”(product of Water Research Institute, Ostrava, Czech Republic). Two outlying points were removed at the evaluation.

The determination of NOEC values was done by ANOVA (Analysis of Variance) analysis. Used ANOVA method is the part of statistical software QC.Expert 2.5 © 1998-2000 (TriloByte, Czech Republic).

The nominal concentrations of the test substance were used for all evaluations and results.