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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
September 2 to 3, 1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Remarks:
Translated report says "GLP Compatible"
Analytical monitoring:
not specified
Details on sampling:
No details
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: direct dissolution in water
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: not specified
- Source: National Institute for Environmental Studies of Environment Agency

- Method of breeding: not specified
- Feeding during test
- Food type: chlorella vulgaris
- Amount: 2x10^7 cells per feeding
- Frequency: once per day
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
none specified
Hardness:
39 mg/L (reported as solidity). Alkalinity - 35 mg/L. Ca:Mg ratio = 2.8, Na:K ratio = 2.5
Test temperature:
19 +/-1 °C
pH:
Maintained at 7.6
Dissolved oxygen:
8.9 to 9.0 mg/L
Salinity:
Not applicable
Conductivity:
No details
Nominal and measured concentrations:
Control, 100, 180, 320, 560 and 1000 mg/L (nominal concentrations)
Details on test conditions:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:dechlorinated tap water

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hurs light : 8 hours dark
- Light intensity: 500 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- immobilisation check after 24 hours exposure

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study
- Test concentrations: 0.1, 1.0, 10, 100 & 1000 mg/L
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
no
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
mobility
Details on results:
No immobile Daphnia were observed in either the control or test treatments.
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
Not applicable
Validity criteria fulfilled:
not specified
Conclusions:
The 24 hour EC50 for Daphnia magna exposed to pentaerythritol is greater than 1000 mg/L (the highest nominal exposure concentration tested). No immobility was observed during the study, therefore the corresponding no-observed effect concentration (NOEC) was 1000 mg/L.
Executive summary:

The 24 hour EC50 for Daphnia magna exposed to pentaerythritol is greater than 1000 mg/L (the highest nominal exposure concentration tested). No immobility was observed during the study, therefore the corresponding no-observed effect concentration (NOEC) was 1000 mg/L.

Description of key information

An experimental study which investigated the acute toxicity of pentaerythritol to Daphnia magnas howed no immobility of organisms in 1000 mg/L exposure concentrations of the test substance throughout the 24 hour exposure period. The 24 hour EC50 for D. magna exposed to pentaerythritol was therefore concluded to be >1000 mg/L (the highest nominal exposure concentration tested), therefore the corresponding no-observed effect concentration (NOEC) was 1000 mg/L (Fukuoka, 1993).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1 000 mg/L

Additional information