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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Description of key information

The chronic toxicity of 4 -Hydroxydiphenylamine to Daphnia magna was investigated in a static test according to Council Regulation (EC) No 440/2008, Method C.20 'Daphnia magna Reproduction Test'' (2008), which equals to the OECD Test guideline 211 (Daphnia magna Reproduction Test; 1998). After 21 days a NOEC of 0.028 mg/L and a LOEC of 0.0.87 mg/L was determined (Currenta, 2010).  A further test performed with p-Hydroquinone yieled a NOEC of 0.0029 mg/L, expressed in 6PPD equivalents of 0.007 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.007 mg/L

Additional information

4-Hydroxydiphenylamine (4 -HPDA) is the hydrolysis product from N-(1,3-dimethylbutyl)-N'-phenylbenzene-1,4-diamine (6PPD). A secondary hydrolysis product is p-hydroquinone.


The lowest effect value in long-term tests was found for p-hydroquinone (0.007 mg/L, expressed in 6PPD equivalents) which is lower than the value for 4-HDPA (0.028 mg/L).Although p-hydroquinone is a secondary hydrolysis product of 6PPD, it was chosen as a source of key value as it shows the lowest effect concentrations of the degradation products and is therefore the worst case.