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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Not known`
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report Date:
1970

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
no
Test type:
other:
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Carbon monoxide
- Substance type: gas
- Physical state: 5% CO in air
- Analytical purity: Not stated
- Impurities (identity and concentrations): Not stated
- Purity test date: Not stated
- Lot/batch No.: Not stated
- Expiration date of the lot/batch: Not stated
- Stability under test conditions: Not stated
- Storage condition of test material: Not stated

Test animals

Species:
rat
Strain:
Crj: CD(SD)
Sex:
male

Administration / exposure

Route of administration:
inhalation: gas
Type of inhalation exposure:
whole body
Vehicle:
air
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
ca. 4 h
Remarks on duration:
single exposure
Concentrations:
1300, 1375, 1960, 2000
No. of animals per sex per dose:
6/dose
Control animals:
no

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
ca. 1 300 ppm
Based on:
other:
95% CL:
ca. 1 226 - ca. 1 378
Exp. duration:
4 h
Remarks on result:
other: single exposure

Any other information on results incl. tables

Mortality:

Conc.

Mortality
(Died/no. treated)

1300

3/6

1375

5/6

1960

6/6

2200

6/6

Pathology:

Gross: All internal organs were bright red in color. Lungs were heavy. Skin had pink flush, which was particularly noticeable when skin was separated from carcass.

Histopathology: slight focal hyperinflation of alveoli.

Applicant's summary and conclusion

Interpretation of results:
Category 3 based on GHS criteria
Remarks:
Migrated information
Conclusions:
The LC50 for carbon monoxide for a four hour exposure of young adult male rats was deemed 1300 ppm.
Executive summary:

The study consisted of 4 groups of 6 male rats dosed with CO for a single 4 hours exposure at doses of 1300, 1375, 1960 and 2200ppm, via whole body inhalation. Necropsy followed on day 14.

Clinical signs of toxicity during exposure: irregular breathing, red color to ears and eyelids, no response to sound, slight lacrimation at 1300, 1375 and 1960 ppm

Clinical signs of toxicity post exposure: red colour remained in ears for a short time, slight weight loss first day followed by normal weight gain, sensitivity to touch.

Gross: All internal organs were bright red in color. Lungs were heavy. Skin had pink flush, which was particularly noticeable when skin was separated from carcass.

Histopathology: slight focal hyperinflation of alveoli.

The LC50 for carbon monoxide for a four hour exposure of young adult male rats was deemed to be 1300 ppm.