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Diss Factsheets

Administrative data

Endpoint:
toxicity to other aquatic vertebrates
Type of information:
other: Publication
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented publication
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Toxic effects of endocrine disruptors on freshwater sponges: common developmental abnormalities
Author:
Hill, M. et al.
Year:
2002
Bibliographic source:
Environmental Pollution 117:295-300

Materials and methods

Principles of method if other than guideline:
Method: other
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethylbenzene
EC Number:
202-849-4
EC Name:
Ethylbenzene
Cas Number:
100-41-4
Molecular formula:
C8H10
IUPAC Name:
ethylbenzene
Details on test material:
IUCLID4 Test substance: other TS
Ethylbenzene
CAS No. 100-41-4
Purity: no data
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
No data

Test solutions

Vehicle:
not specified
Details on test solutions:
No data

Test organisms

Test organisms (species):
other: other: freshwater sponge - Heteromyenia species and Eunapius fragilis
Details on test organisms:
No data

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
9 d
Post exposure observation period:
No data

Test conditions

Hardness:
No data
Test temperature:
No data
pH:
No data
Dissolved oxygen:
No data
Salinity:
No data
Nominal and measured concentrations:
0.03, 0.3 and 3 mg/l
Details on test conditions:
According to the author, no official standards mentioned.
The toxic effect of ethylbenzene was examined on growth and development of two species of freshwater sponge, Heteromyenia spec. and Eunapius fragilis.
Test concentrations: 0.03, 0.3 and 3 mg/l
Obeservation period: 3, 6 and 9 days

Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
9 d
Dose descriptor:
EC100
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: germination
Duration:
9 d
Dose descriptor:
LOEC
Effect conc.:
0.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: abnormal growth
Details on results:
At 0.3 mg/l abnormal growth in Heteromyenia species was observed. Sponges initiated germination, but produced a tight cluster (or tube) of cells that seemed to be exuded from the gemmule. Germination was completely inhibited at 3 mg/l
Results with reference substance (positive control):
No data
Reported statistics and error estimates:
No data

Any other information on results incl. tables

None

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
At 0.3 mg/l abnormal growth in Heteromyenia species was observed. Sponges initiated germination, but produced a tight cluster (or tube) of cells that seemed to be exuded from the gemmule. Germination was completely inhibited at 3 mg/l
Executive summary:

The toxic effect of ethylbenzene was examined on growth and development of two species of freshwater sponge, Heteromyenia spec. and Eunapius fragilis.Test concentrations: 0.03, 0.3 and 3 mg/l Obeservation period: 3,6 and 9 days.

At 0.3 mg/l abnormal growth in Heteromyenia species was observed. Sponges initiated germination, but produced a tight cluster (or tube) of cells that seemed to be exuded from the gemmule. Germination was completely inhibited at 3 mg/l