4,4'-methylenediphenyl diisocyanate

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable study report with restrictions due to limited reporting

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

A one-week acute intraperitoneal toxicity test was carried out to decide the dose level for the micronucleus test. Groups of 5-6 male mice were treated with the test material at different dose levels in 3 series of experiments, and LD50 was estimated from the number of deaths.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4,4'-methylenediphenyl diisocyanate

EC Number:

202-966-0

EC Name:

4,4'-methylenediphenyl diisocyanate

Cas Number:

101-68-8

Molecular formula:

C15H10N2O2

IUPAC Name:

1-isocyanato-4-[(4-isocyanatophenyl)methyl]benzene

Details on test material:

- Name of test material (as cited in study report): 4,4'-Diphenylmethane diisocyanate
- Analytical purity: not reported
- Impurities (identity and concentrations): not reported

Test animals

Species:

mouse

Strain:

ICR

Sex:

male

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: DMSO and corn oil

Details on exposure:

- Preparation of test material: MDI is insoluble in water and reacts with it to form polymers. Therefore, the test material was dissolved in a small amount of dimethylsulfoxide (DMSO), the first solvent, which does not contain water, and then it was suspended in the sterilized corn oil, the second solvent.The preparation was made separately for each test and used immediately.

Doses:

Experiment I: 10, 20, 100 and 500 mg/kg bw;
Experiment II: 133, 200, 300 and 450 mg/kg bw;
Experiment III: 89, 133 and 200 mg/kg bw

No. of animals per sex per dose:

5 in Experiment I
6 in Experiment II and III

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 100 mg/kg bw

Mortality:

see free text

Any other information on results incl. tables

Experiment No.	Dose levels (mg/kg bw)	No. of animals died/ No. of animals treated	Time of death (day)
			0-1	1-3	3-5	5-7
1	500	5/5	0	3	2	0
	100	0/5	0	0	0	0
	20	0/5	0	0	0	0
	10	0/5	0	0	0	0
2	450	4/6	0	3	1	0
	300	5/6	0	2	1	2
	200	5/6	0	4	1	0
	133	4/6	0	2	0	2
3	200	5/6	0	2	3	0
	133	4/6	0	1	1	2
	89	0/6	0	0	0	0