2-chloro-4-nitrotoluene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Materials and methods

Principles of method if other than guideline:

Method: other: Verfahrensvorschlag (F.1.1) "Letale Wirkung beim Zebrabaerbling Brachydanio rerio" (LC0, LC50, LC100; 48 - 96 Stunden) des Umweltbundesamtes, Stand 01.06.1982

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chloro-4-nitrotoluene

EC Number:

204-501-7

EC Name:

2-chloro-4-nitrotoluene

Cas Number:

121-86-8

Molecular formula:

C7H6ClNO2

IUPAC Name:

2-chloro-1-methyl-4-nitrobenzene

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

-stock solution: 1g of test substance was dissolved in 1 g ethanol, made up to 100 mL with dest water and treated with an ultra turrax
-to produce the test concentrations a sample of stock solution was taken under agitate (flocculation)

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Length at study initiation (length definition, mean, range): 30 +-5 mm

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

-total hardness ca. 2 mmol (Ca + Mg)
-Ca/Mg-ratio 4:1

Test temperature:

18.2-20.9°C

pH:

6.3-7.3

Dissolved oxygen:

6.7-9.3

Nominal and measured concentrations:

31, 32, 33, 34, 35, 40 mg/L

Details on test conditions:

-Test vessel: 8.4 L with 5 L conditioned tap water
-loading rate: 10 animals/vessel/concentration
-no feeding during exposure
-aeration: moderate (3 blebs per second)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

34 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

35 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

RS-Freetext:
LC50 could not be determined due to steepness of dose-effect relationship

Applicant's summary and conclusion

Executive summary:

The acute toxicity of 2-chloro-4-nitrotoluene to fish (brachydanio rerio) was determined in a static mortality test according to a national standard method (Verfahrensvorschlag F.1.1, des Umweltbundesamtes, 1982). After 96 hours of exposure a LC0 value of 34 mg/L and a LC100 value of 35 mg/L was optained,(Bayer, 1984).