Chrome antimony titanium buff rutile

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Rating in accordance with OECD SIDS (2002)

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Reference 2

Reference Type:

other: Translation of original report

Title:

Unnamed

Year:

1997

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: DIN 38412 Part 27 (draft)

Principles of method if other than guideline:

Robra, K .H., gwf. Wasser-Abwasser 117, 80-86, 1976

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Chrome antimony titanium buff rutile

EC Number:

269-052-1

EC Name:

Chrome antimony titanium buff rutile

Cas Number:

68186-90-3

Molecular formula:

(Ti, Sb, Cr) O2

IUPAC Name:

manganese(4+) trititanium(4+) pentaantimony(3+) chromium(3+) nickel(2+) octadecaoxidandiide

Test material form:

solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test solution stirred for 17 h at 19.85 °C and than used as dispersion (insoluble in water) in the oxygen consumption test.

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: yes
- Method of cultivation: The stemculture is taken up in 0.9 % strength NaCl solution.
Batches of 200 mL of culture fluid (25 g/L of nutrient broth I) are inoculated with cell suspension under sterile conditions.
The cells are harvested after 16 ± 2 h of shaking on a rotary shaker at 22 °C and 200 r.p.m.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Test conditions

Test temperature:

25 °C

pH:

7.2 - 8.0

Nominal and measured concentrations:

control, 1250, 2500, 5000, 10000 mg/L (nominal)

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total volume 100 mL
- 5 mL of glucose (198 g/L)
- 95 mL of the test substance in the given concentrations
- 2 - 3 mL of bacterial suspension
(the volume of the suspension must be chosen such that the oxygen consumption of the blank value is 1.5 - 2.5 mg/L*min)

The assay batch was aerated for 30 min at 25 °C. The decline of the dissolved oxygen concentration was measured in a flow cell.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

dispersed

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Key result

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

dispersed

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Any other information on results incl. tables

The results show the nominal concentrations of the test substance that inhibited oxygen consumption of the microorganisms.

 

Concentration [mg/L]	Mean oxygen consumption [% of control]
1250	101.1
2500	107.7
5000	101.1
10000	94.5

Applicant's summary and conclusion

Conclusions:

Chrome antimony titanium buff rutile is not toxic to the microorganisms in a test according to the German standard method DIN 38412 Part 27 with test suspensions prepared at nominal concentrations far exceeding its water solubility. The corresponding EC50 and EC10 for inhibition of oxygen consumption are both estimated with > 10000 mg/L (nominal).