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EC number: 205-438-8 | CAS number: 140-88-5
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 Apr 1980 - 23 Sep 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study report which meets basic scientific principles, acceptable with restriction. Neither Escherichia coli strain WP2 uvrA pKM101a nor S. typhimurium strain TA102 were employed in this study for the detection of oxidising mutagens and cross-linking agents.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Ames test (Ames et al., Mutation Research 31: 347, 1975)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Ethyl acrylate
- EC Number:
- 205-438-8
- EC Name:
- Ethyl acrylate
- Cas Number:
- 140-88-5
- Molecular formula:
- C5H8O2
- IUPAC Name:
- ethyl acrylate
- Details on test material:
- - Name of test material (as cited in study report): Ethyl acrylate
- Analytical purity: no data
Constituent 1
Method
- Target gene:
- Histidin auxotrophy
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver from Aroclor 1254 pre-induced rats
- Test concentrations with justification for top dose:
- 0.001, 0.01, 0.1, 1.0, and 5.0 µl/plate for strains TA1535, TA1537, TA98, and TA100
.
Test repeated with 1.0, 2.5, 5.0, 6.0, and 7.5 µl/plate for strain TA1535 and with 0.001, 0.01, 0.1, 1.0, and 5.0 µl/plate for TA 98. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Anthramine (TA 1535, TA 1537, TA 100), 2-Acetamidofluorene (TA 98)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Mean His (+) revertants/plate:
Main Test:
Concentration [µL/plate] |
TA 1535 |
TA 1537 |
TA 98 |
TA 100 |
||||
A |
S |
A |
S |
A |
S |
A |
S |
|
5.0 |
60.3* |
39.3 |
21.7 |
11.0 |
37.0 |
21.3 |
89.7 |
93.3 |
1.0 |
46.7 |
39.0 |
26.0 |
8.7 |
57.7 |
12.0 |
96.3 |
88.7 |
0.1 |
34.7 |
37.7 |
26.0 |
9.3 |
136.7 |
25.7 |
100.0 |
115.7 |
0.01 |
35.0 |
48.7 |
26.7 |
10.7 |
137.7 |
23.7 |
110.7 |
112.7 |
0.001 |
35.3 |
42.7 |
29.7 |
10.3 |
135.0 |
25.3 |
93.0 |
104.3 |
DMSO |
36.0 |
41.9 |
24.4 |
11.3 |
141.4 |
22.7 |
100.2 |
98.8 |
2-AA |
223 |
41.0 |
361.8 |
13.5 |
434.7 |
81.8 |
||
2-AAF |
693.2 |
15.2 |
||||||
Retest 1:
Concentration [µL/plate] |
TA 98 |
|
A |
S |
|
5.0 |
51.0 |
11.7 |
1.0 |
48.7 |
22.7 |
0.1 |
50.7 |
21.7 |
0.01 |
60.0 |
25.7 |
0.001 |
57.3 |
22.0 |
DMSO |
52.8 |
23.1 |
2-AA |
||
2-AAF |
2366.8 |
24.3 |
Retest 2:
Concentration [µL/plate] |
TA 1535 |
|
A |
S |
|
7.5 |
19.7 |
25.3 |
6.0 |
17.3 |
20.0 |
5.0 |
23.3 |
28.7 |
2.5 |
30.0 |
17.0 |
1.0 |
24.3 |
24.0 |
DMSO |
21.5 |
26.3 |
2-AA |
420.2 |
28.2 |
2-AAF |
||
A: Liver fromAroclor 1254 pre-induced rats
S: Saline-buffer control mixture
2-AA: 2-Anthramine (10 µg/plate)
2-AAF: 2-Acetaamidofluorene (50 µg/plate)
* difference between the number of revertants at the test concentration and the solvent control is significant; p 0.05
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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