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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003-05-1 to 2003-05-05
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
Study well documented, followed guideline, with analytical.
Justification for type of information:
Category Approach; test material is reference substance LAS. LAS provides suitable read across for LAB Sulfonic Acids as both form the identical chemical species in aqueous solutions at neutral (physiological) pH, namely, the LAS ion (C10-13linear alkyl benzene-SO3-) and would be expected to have similar eco-toxicological properties.
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report date:
2006

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
no
Remarks:
This study was not conducted under GLP but is a guideline study, of high scientific quality with considerable documentation.

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
EC Number:
270-115-0
EC Name:
Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
Cas Number:
68411-30-3
Molecular formula:
Not applicable for UVCB
IUPAC Name:
sodium 4-undecylbenzenesulfonate
Details on test material:
- Name of test material: LAS Pantera (SS1147.01)
- Molecular weight: LAS Pantera: 318.12
- Substance type: Pure active substance
- Physical state: Liquid
- Stability under test conditions: Not available
- Storage condition of test material: At room temperature
- Color: Brown

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: Samples were taken from all test concentrations at 0 and 72h (fresh solutions), and at 24 and 96h (24 h old solutions).
- Sampling method: Samples were collected and immediately preserved with 30% (v/v) methanol and an internal standard of deuterated C12-LAS was added to facilitate quantitation.
- Sample storage conditions before analysis: Not available

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solutions of LAS material were prepared in high quality water at 1 g/L. Stock solutions were neutralized to a pH of 8.6 using NaOH. Test solutions were prepared by dilution of the stock solution in the dilution water (a blend of well and reverse osmosis water).
- Controls: A blend of well water and Reverse Osmosis Water.
- Evidence of undissolved material: No

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: Fathead Minnow
- Strain: Not available
- Source: Breeders obtained from Aquatic Research Organisms (ARO, Hampton, NH, USA). Test organisms were reared from eggs from the in-house breeding culture.
- Age at study initiation: Not specified.
- Length at study initiation: Average length: 2.74 cm, SD-0.264cm, Range-2.30-3.22 cm
- Weight at study initiation: Average weight: 0.189 g, SD-0.0615g, Range-0.101-0.317 g
- Method of breeding: In-house breeding; continuous batch culture; 20 L culture vessels; blended water (mix of well and RO water) was culture water; Temp 20.6-21.4°C; DO 7.7-8.9; pH 7.26-8.21; Food - live brine shrimp, flake food; 16:8 hr light:dark.
- Feeding during test: No


ACCLIMATION
- Acclimation period: Test organisms were bred/maintained under the same conditions as the test. The length of the 'acclimation' period was therefore the same as the age of the test organisms.
- Acclimation conditions: Same as test
- Type and amount of food: Live brine shrimp, flake food
- Feeding frequency: Twice daily
- Health during acclimation (any mortality observed): No mortality in the week prior to testing

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
Not available. But the blend of well and RO water has a hardness of ~180 mg/L as CaCO3.
Test temperature:
LAS-Pantera: range 20.6-21.4 Degree C
pH:
LAS-Pantera: range 7.26-8.27. Details on pH measurements are provided in a Table below.
Dissolved oxygen:
LAS-Pantera: range 7.3-8.5 mg/L. Details on DO measurements are provided in a Table below.
Nominal and measured concentrations:
Nominal concentrations for LAS-Pantera: 0, 0.25, 0.5, 1, 2, 4, 8, 16 mg/L were based on the active ingredient in the test material.
Measured concentrations for LAS-Pantera: 0, 0.24, 0.57, 0.98, 2.1, 3.96, 7.61, 13.9 mg/L were based on the active ingredient.
Measured concentrations were 87-114% of nominal.
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: open
- Material: 19 L Glass aquaria, 10 L of solution
- Aeration: Not available
- Renewal rate of test solution: Daily renewal (24,48,72 h)
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2 replicates
- No. of vessels per control (replicates): 2 replicates
- Biomass loading rate: 0.19 g/L.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: South west well water and reverse Osmosis water blended together
- Total organic carbon: Not available
- Particulate matter: Not Available
- Metals: Not Available
- Pesticides: Not Available
- Chlorine: Not Available
- Alkalinity: Not Available
- Ca/mg ratio: Not Available
- Conductivity: 370-425 umhos/cm for LAS-Pantera
- Culture medium different from test medium: No
- Intervals of water quality measurement: Temperature, DO, pH and conductivity measured daily in control and each test concentration.

OTHER TEST CONDITIONS
- Adjustment of pH: Not applicable
- Photoperiod: 16 h light :8 h dark
- Light intensity: 7-10uE/m2.s

EFFECT PARAMETERS MEASURED: Mortality was observed at 24, 48, 72 and 96 h .

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0
- Range finding study: No, but the test concentrations were based on SAR predictions.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.88 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% CL: 2.10-3.96 mg/L
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
2.88 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% CL: 2.10-3.96 mg/L
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
2.88 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% CL: 2.10-3.96 mg/L
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
2.88 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% CL: 2.10-3.96 mg/L
Details on results:
- Behavioural abnormalities: None
- Observations on body length and weight: No significant change in body weight and length
- Other biological observations: None
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No
Reported statistics and error estimates:
Mortality results were summarized and 96 hr LC50 estimates and 95% confidence limits were generated using the Binomial Probability Method.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Table 1: % Mortality in Acute Toxicity Test (study #45725; LAS Pantera) (20 fish/test concentration)

Time (hr)

Active Ingredient of Test Substance (measured concentration; mg/L)

0

0.24

0.57

0.98

2.1

3.96

7.61

13.9

24

0

0

0

0

0

100

100

100

48

0

0

0

0

0

100

100

100

72

0

0

0

0

0

100

100

100

96

0

0

0

0

0

100

100

100

Table 2: Acute Toxicity of 7 LAS Substances to Fathead Minnow (Study # 45725)

Test Material 96 hr LC50 (mg/L) 95% CL (in mg/L)
C10 LAS 16.6 15.0 – 18.3
C11 LAS 7.08 6.76 – 7.42
C12 LAS 1.63 1.49 – 1.78
C13 LAS 0.63 0.55 – 0.71
C14 LAS 0.26 0.23 – 0.30
LAS Veneco (C10,12,14) 2.22 2.02 – 4.35
LAS Pantera (C10,11,12,13) 2.88 2.10 – 3.96

Results are based on measured test concentrations.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
(Criteria of OECD 203: Mortality of controls <10%, conc. of dissolved oxygen >60%, exposure concentrations based on measured concentrations)
Conclusions:
The 96 hr LC50 of LAS Pantera to Fathead minnows was 2.88 mg a.i./L, based on measured concentrations of active ingredient.
Executive summary:

In a 96 h static renewal acute toxicity study, Fathead minnow (Pimephales promelas) were exposed to LAS Pantera at nominal concentrations of 0 (control), 0.25, 0.5, 1, 2, 4, 8, and 16 mg a.i./L. Measured concentrations were 0 (control), 0.24, 0.57, 0.98, 2.1, 3.96, 7.61, and 13.9 mg a.i./L. Measured concentrations were 87 -114% of nominal.

The 96 h LC50 value, based on measured test concentrations, for LAS- Pantera was 2.88 mg a.i./L with 95% confidence interval of 2.10 to 3.96 mg a.i./L.

This toxicity study is classified as acceptable and satisfies the guidelines requirement for the OECD 203 fish acute toxicity study.

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