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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

The experimental 96 hour EL50 for Pimephales promelas exposed to cyclohexane was 4.35 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
4.35 mg/L

Additional information

The study selected as key by Geiger et al. (1987) uses a flow-through system with analytical monitoring, a standard test species (Pimephales promelas) and standard exposure duration (96 hours). The method used in this paper is equivalent to OECD 203. The only minor deviation is that behavioural data was not recorded. We do not consider that this deviation significantly alters the validity of this study. A flow-through system with analytical monitoring was used and all validity criteria of OECD 203 were met. This study was chosen in preference to other studies identified as it was used as the key study in the EU RAR (2004) for cyclohexane, it was the only study using a flow-through system and reported the most conservative value for fish.