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Toxicity to aquatic algae and cyanobacteria

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Description of key information

The acute toxicity of Sugar Factory Lime (SFL) to algae has been investigated in a GLP study performed in accordance with OECD Guideline 201. The 72 h EC50 was >100 mg/L based on the water accommodated fraction.
The results from this study demonstrate that SFL is not acutely toxic to aquatic algae.

Key value for chemical safety assessment

Additional information

The key study [Degussa (2007d)] was performed to OECD Guideline 201 and in accordance with GLP and was therefore assigned a reliability of 1. The study assessed the acute toxicity of Sugar Factory Lime (SFL) to Desmodesmus subspicatus (green algae) in a limit test. Since the test substance is a multi-component substance and is poorly soluble in water, the test was performed with the water-accommodated fraction (WAF) of 100 mg SFL/L medium. No toxic effects were seen at this test concentration. Hence, the 72 h EC50 was >100 mg/L based on both the growth rate and yield and the 72 h NOEC was 100 mg/L.

The study for SFL is supported by an acute algal toxicity study performed with calcium carbonate (nano) [Vryenhoef & Mullee (2010)]. The study assessed the acute toxicity of calcium carbonate (nano) to Desmodesmus subspicatus (green algae) in a limit test performed with a measured concentration of 14 mg/L. No toxic effects were noted at the concentration tested. Hence, the 72 h EC50 for calcium carbonate (nano) was found to be >14 mg/L and the NOEC was 14 mg/L. The concentration of calcium carbonate (nano) that might cause acute toxicity is therefore greater than the maximum solubility of calcium carbonate in water.