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No terrestrial studies are available for sugar factory lime (SFL). However, three reliable OECD guideline studies are available which demonstrate the effects of calcium carbonate (nano) on terrestrial species. The species studied were Eisenia foetida(earthworm), plants (soybean, tomato and oat) and soil microorganisms. The tests were all performed as limit tests using a calcium carbonate (nano) concentration of 1000 mg/kg. The results of these studies demonstrate that the concentration of calcium carbonate (nano) that might cause toxicity to terrestrial species is greater than the highest concentration tested and is therefore greater than the maximum solubility of calcium carbonate in water.

The results from studies performed with calcium carbonate (nano) are directly applicable to SFL. On this basis, SFL is not acutely toxic to terrestrial organisms and PNECs for the terrestrial compartment have not been derived.