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Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted to GLP.

Data source

Reference Type:
study report
Report date:

Materials and methods

Principles of method if other than guideline:
ASTM, 1984b. Proposed New Standard Practice for conducting Life-cycle Toxici ty Tests with Saltwater Mysids. Subcommittee E.47.01, May, 1984.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
- Name of test material (as cited in study report): acrylamide monomer (2-propenamide; CAS No. 79-06-1)
- Physical state: whi te crystalline solid
- Analytical purity: Ultra Pure Electrophoresis Grade 99.9%
- Lot/batch No.: Lot 44569

Sampling and analysis

Analytical monitoring:

Test solutions

Details on test solutions:
SOLVENT /CARRIER: Deionized water

Test organisms

Test organisms (species):
Americamysis bahia (previous name: Mysidopsis bahia)
Details on test organisms:
- Common name: Mysid shrimp
- Strain/clone: Mysidopsis bahia
- Source: Bionomics Culture,
- Age of parental stock : ≤ 26 hours old
The test organisms were held in natural seawater at 30 parts per thousand (0/00) salinity and 25°C prior to distribution into the test aquaria.

Study design

Test type:
Water media type:
Limit test:
Total exposure duration:
28 d

Test conditions

Not measured
Test temperature:
22.9-26.9 °C (mean 24.8±0.6 °C)
Dissolved oxygen:
5.3-7.2 mg/L ( >80% of saturation)
mean 30± 1 0/00 (28-31 0/00)
Nominal and measured concentrations:
Targeted nominal test concentrations: 0.09, 0.19, 0.38, 0.78, 1.50, 3.0 and 6.0 mg/L
Mean measured test concentrations: 0.06, 0.12, 0.24, 0.45, 1.02, 2.04 and 4.40 mg/L
Details on test conditions:
- Test vessel:
- Material: aquarium with fill volume of 9 L . The retention chambers were glass Petri dishes to which a 15-centimeter high nylon screen collar was attached with silicone sealant.
- Aeration: no
- Type of flow-through: proportional diluter
- Renewal rate of test solution (frequency/flow rate): Each diluter was constructed to deliver 0.5 L/cycle/test aquarium at a dilution rate of 50. The average number of diluter cycles was approximately 6.1 or 6.7 per hour, providing approximately 8-9 volume replacements per aquarium every 24 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Feeding during test: live brine shrimp (Artemia salina) nauplii daily
- Source: Seawater (natural, filtered from Big Lagoon)
- Intervals of water quality measurement: The results of routine water quality analyses for metals, nutrients, pesticides, PCB's and petroleum hydrocarbons. Test dilution water quality was also biologically monitored in the laboratory through the maintenance of continuous cultures of mysids.
Reference substance (positive control):

Results and discussion

Effect concentrationsopen allclose all
28 d
Dose descriptor:
Effect conc.:
2.04 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
parental F0
28 d
Dose descriptor:
Effect conc.:
2.04 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
Offspring F1
28 d
Dose descriptor:
Effect conc.:
4.4 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
Details on results:
- Mortality of parent animals: from 8 to 22% in test concentrations >2.04 mg/L. Mortalities at these concentrations were not significantly different from the control mortality of 22%. At test termination, mortality in the highest concentration tested, 4.40 mg/L, reached 45% which was statistically greater than the control.
- No. of offspring produced per day per female: The mean number of offspring produced per female reproductive day ranged from 0.13 to 0.50 with no apparent correlation to acrylamide concentration; mean number of offspring in the control per female reproductive day was 0.33.
- Body length and weight of parent animals: Average dry weights of male mysids ranged from 0.42 mg at 4.40 mg/L acrylamíde to 0.55 mg in the control seawater. Male mysids exposed to ≥0.12 mg/L acrylamide had dry weights which were significantly less when statistically compared to the dry weights of control male mysids. Average dry weights of female mysids ranged from 0.47 mg at 4.40 mg/L acrylamide to 0.62 mg in the control and 0.24 mg/L test concentration. Female mysids exposed to ≥1.02 mg/L acrylamide had dry weights which were significantly less when statistically compared to the dry weights of control female mysids. Dry weight data of females probably do not accurately reflect growth, because of the possible presence of Artemia nauplii in their brood pouches. As Artemia were not removed prior to weight measurements, use of these data for setting criteria standards is not recommended by this laboratory.
- Number of males and females (parental): 40 per concentration
- Time to first brood release or time to hatch: There was no significant delay in time to release of the first brood at any test concentration. Time to first brood release in all replicate control and test aquaria ranged from 15 to 24 days with 84% of all first brood releases occurring between 15 and 20 days of exposure. Mean times to first brood release were not apparently concentration-related and ranged from 16 days in 0.24 mg/L to 21 days in 1.02 mg/L acrylamide

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Mysid reproduction was not adversely effected at any tested concentration. The NOEC was determined to be 2.04 mg/L for both parental (F0) and offspring (F1) survival.