Acrylamide

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Acrylamide

EC Number:

201-173-7

EC Name:

Acrylamide

Cas Number:

79-06-1

Molecular formula:

C3H5NO

IUPAC Name:

prop-2-enamide

Details on test material:

- Name of test material (as cited in study report): ACRYLAMIDE 50 ST
- Substance type: organic
- Physical state: Colourless liquid
- Composition of test material, percentage of components: 50% in aqueous solution.
- Lot/batch No.: 5069
- Storage condition of test material: In dakness and room temperatue.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Green Algae
- Strain: Selenastrum capricornutum (CCAP 278/4)
- Source (laboratory, culture collection): AQUARIUM DE TROUVILLE (17 rue de Paris, 14360 TROUVILLE).
- Age of inoculum (at test initiation): approximately 72-hours
- Method of cultivation: the sub-culture used for the algal inoculation was made in the same culture medium as that used for the test, and kept under the same conditions as those employed for the test (culture medium, lighting, temperature, sting). Contemporaneously to the start of the test, pre-culture cell concentration was measured and the volume to be used for the inoculation of test and control media was determined.
ACCLIMATION
- Acclimation period: none reported
- Culturing media and conditions: same as test
- Any deformed or abnormal cells observed: none reported

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Hardness:

40 mg/L

Test temperature:

24.0-24.5°C

pH:

6.95-8.84

Dissolved oxygen:

Not reported

Nominal and measured concentrations:

Nominal : 10, 18, 32, 56 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: Erlenmeyer flasks of 250 ml capacity, provided with a cotton-wool plug.
- Aeration: no
- Initial cells density: 9,600 cells per millilitre
- Control end cells density: 3,263,333 per millilitre
- No. of vessels per concentration (replicates): 3
- No. of vessels per vehicle control (replicates): 3
GROWTH MEDIUM and TEST MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: sterile tap water
- Culture medium different from test medium: No
- Total organic carbon: 0 mg/l
- Particulate matter: 0 mg/l
- Salts: Ca(NO3)2.4 H2O = 40mg/L; KNO3 = 100mg/L, MgSO4.7 H2O = 30Mg/L; K2HPO4 = 40mg/L; CuSO4.5 H2O = 0.015mg/L; (NH4)6.Mo7O24.4 H2O = 0.030mg/L; ZnSO4.7 H2O = 0.030mg/L; CoCl2.6 H2O = 0.030mg/L; Mn(NO3)2.4 H2O = 0.030mg/L; C6H8O7.H20 = 0.030mg/L; H3BO3 = 0.030mg/L; Ammonium iron III citrate (Fe2O3 content = 29-32 %) = 0.8125mg/L; FeSO4.7 H2O = 0.3125mg/L; FeCl3.6 H2O = 0.3125mg/L
- Pesticides: none
- Chlorine: none reported
- Alkalinity: not reported
- Ca/mg ratio: not reported
- Conductivity: not reported
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: readjusted with HCl 0.1N.
- Photoperiod: continuous white lighting
- Light intensity and quality: artificial lighting 7180 - 7230 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations:24 ,48 and 72 hours after the start of the test, the cell concentration was measured for each control and test flask by direct counting of cells with microscope.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10, 18, 32, 56 and 100 mg

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

IC50

Effect conc.:

67.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 2

Duration:

72 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

56 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Any other information on results incl. tables

Inhibitions íif the growth in comparison with the control group (lA as %)

Test substace nomina! concentration (mg/L)	Inhibition of the cell growth (IA ) in 72 hours %
10	-30
18	- 18
32	- 15
56	43
100	64

IA (%) = [(A control - A concentration) / A control] x 100

Inhibitions of the growth rate in comparison with the control group (lµ., as %)

Test substace nominal concentration (mg/L)	Inhibition of the growth rate (Iµ) in 72 hours ( %)
10	-5
18	-3
32	-2
56	14
100	26

Iµ (%) = [(µ control - µ concentration) / µ control ] x 100

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Cell concentration in the control group increased by a factor of at least 16 within 3 days as required: It increased by a factor of 340 during the test period.

Conclusions:

Under the test conditions the ICA50 (growth inhibition) of ACRYLAMIDE 50 ST to green algae Selenastrum capricornutum was 67.7 mg/l and the ICµ50 (growth rate inhibition) was greater than 100 mg/l. The No Observed Effect Concentration was NOEC = 32 mg/l. Therefore, for acrylamide the ICA50 (growth inhibition) is 33.85 mg/l, the ICµ50 (growth rate inhibition) is greater than 50 mg/l and the NOEC is 16 mg/l.