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EC number: 231-111-4 | CAS number: 7440-02-0
The test item Nickel metal was assessed for its potential to induce mutations at the HPRT locus using V79 cells of the Chinese Hamster.
The selection of the concentrations was based on data from the pre-experiments. The experiment with and without metabolic activation were performed as a 4 h short-term exposure assay. The test item was investigated in the main experiment at the following concentrations for evaluating mutagenicity:
without metabolic activation:
0.10, 0.25, 0.50, 1.0 and 2.5 mM
and with metabolic activation:
0.05, 0.10, 0.25, 0.50 and 1.0 mM
Precipitation of the test item was noted in the main experiment at concentrations of 2.5 mM (without metabolic activation) and 1.0 mM (with metabolic activation).
Biologically relevant growth inhibition (relative survival < 70%) was observed in the experiment without metabolic activation. The relative survival was 38% for the highest concentration (2.5 mM) evaluated.
No growth inhibition was observed in the experiment with metabolic activation.
In the experiments no biologically relevant increase of mutants was found after treatment with the test item (without and with metabolic activation). All mutant values are within the historical data base of the test facility.
A statistical analysis displayed that at one concentration (0.25 mM) the mutant frequency of the experiment with metabolic activation was significantly increased over those of the solvent controls and a concentration-response relationship was determined in the χ² test for trend. However, since the mutant frequencies at all concentrations were within the historic control range, the statistically significant increase at 0.25 mM was not regarded as biologically relevant.
In the experiment without metabolic activation some of the mutant frequencies were significantly increased over those of the solvent controls, but no concentration-response relationship was determined in the χ² test for trend. However, as the highest evaluated concentrations were within the historic control range, the statistically significant increases in the mid-range were not regarded as biologically relevant.
DMBA and EMS were used as positive controls and showed distinct and biologically relevant effects in mutation frequency.
THIS STUDY WAS REVIEWED BY AN INDEPENDENT EXPERT
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