Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-987-8 | CAS number: 7783-28-0
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Based on a reliable study with diammonium hydrogenorthophosphat, the LC50 in the freshwater fish (Oncorhynchus mykiss) is determined to be >100 mg/L.
Key value for chemical safety assessment
Additional information
In a reliable study performed according to OECD 203 in 2016, 7 rainbow trout (Oncorhynchus mykiss) per concentration were exposed for 96 hours in a semi-static set-up (24 h renewal interval) at 13 -15°C to the following range of nominal test concentrations: 6.25, 12.5, 25, 50 and 100 mg/L. Test concentrations were analytically verified (ICP-MS) and found to a) be in the range 90 -106% of nominal and b) remain stable (based on P content) throughout the 24 h renewal interval (97 -113% relative to initial). Therefore it is justified to base the endpoint on nominal test concentrations. Water quality parameters were maintained throughout the test.
The acute toxicity of DAP to rainbow trout, expressed as LC50, was exceeding the highest tested concentration, thus 96 -h LC50 is >100 mg/L. This value shall be used for chemical safety assessment.
This result is confirmed a.o. by a study performed according to "Standard Methods for the Examination of Water and wastewater (APHA-1985)", where freshwater fish of the carp family (Cirrhinus mrigala/L. Rohita), fry and fingerlings were exposed to diammonium hydrogenorthophosphate under static conditions at different temperatures (21 -36°C). The toxicity of the substance to fish increased with increasing water temperature. According to OECD 203 the recommended temperature for common carp is between 20 -24°C, therefore the LC50s at 21°C are considered most relevant. LC50 (fry; 21°C) = 1700 mg/L, LC50 (fingerlings; 21°C) = 1875 mg/L. Although other studies showed also lower toxicity levels compared to the above study, clear deviations possibly affecting the results were present. In addition, all studies showed toxicity levels >100 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
