Methanol

Genetic toxicity: in vitro

Administrative data

Endpoint:

genetic toxicity in vitro, other

Remarks:

Type of genotoxicity: genome mutation in fungi

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

A comparative study on ethanol and acetaldehyde as inducers of chromosome malsegregation in Aspergillus nidulans.

Author:

Crebelli, R. et al.

Year:

1989

Bibliographic source:

Mutat Res 215: 187-195

Materials and methods

Principles of method if other than guideline:

Determination of chromosomal malsegregation in ascomycetes during mitosis induced by test substance application.

GLP compliance:

not specified

Type of assay:

other: Mitotic recombination in ascomycetes

Test material

Test material information

Constituent 1

Reference substance name:

Methanol

EC Number:

200-659-6

EC Name:

Methanol

Cas Number:

67-56-1

Molecular formula:

CH4O

IUPAC Name:

methanol

Method

Target gene:

not applicable

Species / strain

Species / strain / cell type:

other: Aspergillus nidulans diploid strain P1

Details on mammalian cell type (if applicable):

no data

Additional strain / cell type characteristics:

not specified

Metabolic activation:

without

Test concentrations with justification for top dose:

5.2, 5.6, 6.0, 7.0 % (v/v)

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: no data

Results and discussion

Test results

Key result

Species / strain:

other: Aspergillus nidulans diploid strain P1

Metabolic activation:

without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

7.0 % (lowest concentration to arrest conidial germination)

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

not specified

Additional information on results:

For the phrase selection in field 'Endpoint' the following source information was used as basis:
Type of genotoxicity: genome mutation
Type of study: other: Mitotic recombination in ascomycetes
Guideline:
Species/strain (Method):
- other: Aspergillus nidulans diploid strain P1

Any other information on results incl. tables

There was a concentration-related increase in non-disjunctions (chromosomal malsegregation) with a maximum of about 3 %. No aneuploidies were noted at the lowest test concentration. The result was statistically significant at two concentrations and a dose-response relationship was evident (IPCS/WHO 1997).

No other forms of malsegregation were statistically significant (1/265 cross-over seen at 6.0 %). The survival was reduced in dose-related manner.

Survival and amount of aneuploidy in Aspergillus nidulans after methanol-treatment:

Concentration [%]	Survival [%]	Scored colonies	Aneuploids (yellow segregants)
			counts	[%]
5.2	26	268	0	0
5.6	19	407	6	1.47*
6.0	10	265	8	3.02**
7.0	5	176	0	0
Spontaneous control
0	100	2673	7	0.26

* P<0.01; ** P<0.001

Note: Similar results were obtained with ethanol and other primary aliphatic alcohols. The aneuploidy rate of ethanol reached a maximum of about 10 %.

Applicant's summary and conclusion

Conclusions:

Interpretation of results:
positive