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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 November 1999 - 21 February 2000
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP study according to international guidelines but lacking analytical confirmation of actual test concentrations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid: viscous
Details on test material:
Batch: T4649
Expiry date: 22 April 2001
Storage at 10 to 30 dC

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The analytical method was suitable only for measurement of actual concentrations in samples taken from the stock solutions in acetone. Samples were also taken from the test media at the start and the end of the test. Analysis of the test concentration samples was attempted but resulted in most cases in non-dectable values. Therefore, final evaluation of the results was based on nominal concentrations.

Test solutions

Vehicle:
yes
Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: acetone 0.1 ml/l. Stock solutions in acetone contained 60 or 120 g/L. These were used to prepare the final test solutions.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
23.8 - 25.9 dC
pH:
7.5 - 9.6
Nominal and measured concentrations:
Nominal: 6 mg/L - Stock: 60 g/L - Actual: 54 g/L
Nominal: 12 mg/L - Stock: 120 g/L - Actual: 108 g/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC0
Effect conc.:
ca. 12 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test substance has proven to be non-toxic to fresh-water algae at levels at and above its solubility in water.