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Short-term toxicity to aquatic invertebrates

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Key value for chemical safety assessment

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In a key, static 48 hourDaphnia magnaacute toxicity test (OECD 202, KS=1), five daphnids/ replicate were exposed to the Water Accommodated Fraction (WAF) of heavy cracked fuel oil (MRD-10-579) at nominal concentrations of 0.05, 0.15, 0.45, 1.35 and 4.05 mg/L. The 48 hour EL50 was 0.22 mg/L based on mobility (EMBSI 2012a).

In seven reliable supporting 48-hourDaphnia magnastudies using WAF methodology, the EL50 values of other heavy fuel oil samples varied between 2 and >1000 mg/L (EMBSI, 2008e,f,g,h,i; Shell, 1997a, b).