Calcium 4-chloro-2-(5-hydroxy-3-methyl-1-(3-sulfonatophenyl)pyrazol-4-ylazo)-5-methylbenzenesulfonate

Endpoint summary

Additional information

The acute toxicity of the test item to fish (zebra fish) was determined according to OECD-Guideline for Testing of Chemicals No. 203. A static limit test with a 500 mg/L solution was conducted. The 500 mg/L solution of the test item didn´t show any signs of toxicity nor lethality at all observation time points up to 96 hours.

 

 

The effects of the test item on the growth rate of juvenile zebra fish were determined according to OECD-Guideline 215. A 28 d semi-static test was carried out. No effects were observed up to the highest concentration level tested. Therefore the NOEC = 37.7 mg/L (measured concentrations)

 

 

In the acute immobilization test with Daphnia the effect of different test concentrations of Pigment Yellow 191, was determined according to Directive 84/449/EEC. The test was conducted under static conditions with to test concentrations of 100, 180, 320, 580 and 1000 mg/L (nominal) and the control. DMF was used as solvent. Since the solubility of the substance is far below the EC 50 value of 750 mg/L, the result is treated as not reliable.

 

The toxicity of Pigment Yellow 191 to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD-Guideline 201. The aim of the study was to assess the effects on growth rate and biomass production over a period of 72 h. The study was conducted under static conditions with an initial cell density of approximately 10000 cells/mL. A limit test with a saturated solution was carried out. A solution of 39.8 mg/L test item was prepared. The limit concentration was measured at the beginning and the end of the study. Three replicates were tested for the saturated solution and 6 replicates for the control. Environmental conditions, pH-value and room temperature, were determined to be within the acceptable limits. All effect values are based on the saturated solution of the test item.

No inhibiting effects on biomass growth and specific growth rate were found in the saturated solution. The EC50-values for inhibition of biomass growth (EbC50) and specific growth rate (ErC50) after 72 h were > 39.8 mg/L (mean).

 

The Daphnia magna Reproduction Test (21 d) of the test item was conducted with different test item concentrations according to OECD 211.The test methodwassemi-static. The test solutions were renewed 3 times per week. Aim of the Daphnia Reproduction Test over 21 days was to assess effects on the reproduction capacity and other test item-related effects on parameters such as occurrence of aborted eggs and stillborn juveniles, time of production of first brood, adult mortality, intrinsic rate of natural increase, dry body weight and length of the parent animals.The study resulted aNOEC = 1.88 mg/L (measured).

 

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was performed with the test item. Test system was activated sludge of a municipal treatment plant. The test was performed under static conditions with the nominal limit concentration of 1000 mg/L. The respiration rates of control, reference and test item concentrations were measured after a contact time of three hours, and the inhibitory effects of the test and reference concentrations were determined in comparison to the control respiration rates. No inhibition of the test item was observed at the tested concentration.

In order to check the activity of the test system and the test conditions a reference test was carried out with copper-(II)-sulphate-pentahydrate as reference item and the reference toxicity was determined. The EC50- value for the reference item was 93 mg/L. Validity criteria of the test guideline were fulfilled.

The test item is not toxic at concentrations ≤ 1000 mg/L to activated sludge of a municipal sewage treatment plant.