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EC number: 403-530-4 | CAS number: 129423-54-7 PV-ECHTGELB HGR
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study according to 84/449/EEC and GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Directive 84/449/EEC
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):
A stock solution was prepared by intensive stirring of the test item (0.2 %) in DMF puriss. Fluka (0.02 %). Different test concentrations were prepared by dilution with deionized water.
The following concentrations were tested: 100 mg/L - 1000 mg/L test item, containing 10 mg/L - 100 mg/L DMF - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna STRAUS
- Source: Federal Office of Health , Berlin, Germany; Breeder: Dr. U. Noack-Laboratorien, Sarstedt, Germany
- Method of breeding:
Culture: In 2 L glass vessels at 20 ± 2 °C; vessels ventilated; water change 3 times per week
Culture medium: dilution water according to DIN 38412, part II, 8.2
ACCLIMATION
- Acclimation period: at least 2 h in dilution water
- Type and amount of food: dry food
- Feeding frequency: 2 times per day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
- Test temperature:
- 21.3 °C constant
- pH:
7.29-7.85 (test solutions), 7.42 (control dilution water); 7.71 and 7.76 (control 58 mg/L and 100 mg/L DMF)- Dissolved oxygen:
- 8.2 - 8.5 (test solutions), 8.5 (control dilution water); 8.4 (control 58 mg/L and 100 mg/L DMF)
- Nominal and measured concentrations:
- Nominal concentrations: 100, 180, 320, 580 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers 50 mL, DIN 12 331; 20 mL test solutions
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water (conductivity < 5 µS/cm
OTHER TEST CONDITIONS
- Photoperiod: 12/12 h light/dark cycle
- Light intensity: diffuse light, ca. 10 [µE/m2 x s]
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation at 24 and 48 h
TEST CONCENTRATIONS
- Test concentrations: see above - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate p.a. (Merck)
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 750 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- precipitation of the test substance was observed in all test concentrations approximately 3 h after preparation of the test solution
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Mortality:
- EC0: 0.7 mg/L
- EC50: 1.2 mg/L
- EC100: 2.0 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48 h) = 750 mg/L (nominal). Therefore, the substance has not to be classified for acute aquatic toxicity according to Regulation (EC) No 1272/2008
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS) the effect of different test concentrations of Pigment Yellow 191, was determined according to Directive 84/449/EEC. The test was conducted under static conditions over 48 h. 2 replicates with 10 test organisms respectively were exposed to test concentrations of 100, 180, 320, 580 and 1000 mg/L (nominal) and the control. DMF was used as solvent. A reference test was carried out with potassium dichromate to determine the toxicity of the reference item. The EC50-value of the reference item of 1.2 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS. Water quality parameters pH-value and dissolved oxygen concentration, measured at 0 and 48 h, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.
Since the solubilty of the substance is far below the EC 50 value, the result ist treated as not reliable.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 750 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- precipitation of the test substance was observed in all test concentrations approximately 3 h after preparation of the test solution
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Mortality:
- EC0: 0.7 mg/L
- EC50: 1.2 mg/L
- EC100: 2.0 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48 h) = 750 mg/L (nominal). Therefore, the substance has not to be classified for acute aquatic toxicity according to Regulation (EC) No 1272/2008
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS) the effect of different test concentrations of Pigment Yellow 191, was determined according to Directive 84/449/EEC. The test was conducted under static conditions over 48 h. 2 replicates with 10 test organisms respectively were exposed to test concentrations of 100, 180, 320, 580 and 1000 mg/L (nominal) and the control. DMF was used as solvent. A reference test was carried out with potassium dichromate to determine the toxicity of the reference item. The EC50-value of the reference item of 1.2 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS. Water quality parameters pH-value and dissolved oxygen concentration, measured at 0 and 48 h, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.
Since the solubilty of the substance is far below the EC 50 value, the result ist treated as not reliable.
Referenceopen allclose all
Test concentrations for EC-determination
Test item [mg/L] |
DMF [mg/L] |
Immobilisation 24 h [%] |
Immobilisation 48 h [%] |
||
|
|
Replicate 1 |
Replicate 2 |
Replicate 1 |
Replicate 2 |
100 |
10 |
0 |
0 |
10 |
0 |
180 |
18 |
10 |
0 |
10 |
20 |
320 |
32 |
10 |
10 |
10 |
20 |
580 |
58 |
20 |
10 |
30 |
20 |
1000 |
100 |
50 |
50 |
50 |
60 |
Control dilution water |
0 |
0 |
0 |
0 |
0 |
control 58 mg/L DMF |
0 |
0 |
0 |
0 |
0 |
control 100 mg/L DMF |
0 |
0 |
0 |
0 |
0 |
Test concentrations for EC-determination
Test item [mg/L] |
DMF [mg/L] |
Immobilisation 24 h [%] |
Immobilisation 48 h [%] |
||
|
|
Replicate 1 |
Replicate 2 |
Replicate 1 |
Replicate 2 |
100 |
10 |
0 |
0 |
10 |
0 |
180 |
18 |
10 |
0 |
10 |
20 |
320 |
32 |
10 |
10 |
10 |
20 |
580 |
58 |
20 |
10 |
30 |
20 |
1000 |
100 |
50 |
50 |
50 |
60 |
Control dilution water |
0 |
0 |
0 |
0 |
0 |
control 58 mg/L DMF |
0 |
0 |
0 |
0 |
0 |
control 100 mg/L DMF |
0 |
0 |
0 |
0 |
0 |
Description of key information
In the acute immobilisation test with Daphnia the effect of different test concentrations of Pigment Yellow 191, was determined according to Directive 84/449/EEC. The test was conducted under static conditions with to test concentrations of 100, 180, 320, 580 and 1000 mg/L (nominal) and the control. DMF was used as solvent.
EC50 (48 h) = 750 mg/L
Key value for chemical safety assessment
Additional information
Since the solubilty of the substance is far below the EC 50 value, the result ist treated as not reliable.
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