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EC number: 203-468-6 | CAS number: 107-15-3
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- Ammoniumchloride was ommitted from the medium to prevent nitrification.
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Activated sludge, from an activated sludge plant, was used as inoculum. Secondary activated sludge was obtained from the rzwi Nieuwgraaf in Duiven (1989.02.27). The rzwi Nieuwgraaf is a domestic sewage treatment plant. The sludge was pre-conditioned to reduce the endogenous respiration rates by aeration for a period of three days. The sludge concentration was 2 mg/dm3.
Ammoniumchloride was omitted from the medium to improve the accuracy of the quantication of the biodegradability. - Duration of test (contact time):
- 28 d
- Parameter followed for biodegradation estimation:
- O2 consumption
- Reference substance:
- acetic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 10
- Sampling time:
- 5 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 88
- Sampling time:
- 15 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 95
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Ethylenediamine is degraded 95% in 28 days in the Closed Bottle Test and can be considered readily biodegradable.
- Executive summary:
A study was conducted to determine the ready biodegradability of the test substance in water via measurement of O2 consumption according to EU Method C.4-E, in compliance with GLP. Activated sludge from an activated sludge plant treating predominantly domestic sewage, was used as inoculum. The sludge was pre-conditioned to reduce the endogenous respiration rate by aeration for a period of 3 d. The sludge concentration was 2 mg/dm3. Sodium acetate was used as the reference substance. The test substance degraded 88% in 15 d and at 95% in 28 d. The validity criteria of the study was fulfilled. Under the study conditions, the substance was readily biodegradable (van Ginkel, 1989).
Reference
| Time (days) | oxygen consumption (mg/L) | Biodegradation (%) | ||
| Test | Acetate | Test | Acetate | |
| 0 | 0.0 | 0.0 | 0 | 0 |
| 5 | 0.7 | 3.0 | 10 | 75 |
| 15 | 6.1 | 3.7 | 88 | 93 |
| 28 | 6.6 | 3.8 | 95 | 95 |
Description of key information
Ethylenediamine is readily biodegradable in screening tests.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
A study was conducted to determine the ready biodegradability of the test substance in water via measurement of O2 consumption according to EU Method C.4-E, in compliance with GLP. Activated sludge from an activated sludge plant treating predominantly domestic sewage, was used as inoculum. The sludge was pre-conditioned to reduce the endogenous respiration rate by aeration for a period of 3 d. The sludge concentration was 2 mg/dm3. Sodium acetate was used as the reference substance. The test substance degraded 88% in 15 d and at 95% in 28 d. The validity criteria of the study was fulfilled. Under the study conditions, the substance was readily biodegradable (van Ginkel, 1989).
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