Ethylenediamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Bicarbonate hardness 1.4 meq/l

Test temperature:

20°C

pH:

pH 8 in test medium

Nominal and measured concentrations:

4 replicates, but tested intervals not reported

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

46 mg/L

Basis for effect:

mobility

Remarks on result:

other: SD 4.9

Validity criteria fulfilled:

not specified

Conclusions:

Under the study conditions, the  48 h EC50 was determined to be 46 mg/L.

Executive summary:

A study was conducted to determine the acute toxicity of the test substance to Daphnia magna, according to EU Method C.2 Guideline. Details on test substance concentrations, analytical monitoring and method used were not reported in this study. Also, limited details are available on the results. Therefore adequate evaluation of the study results can not be performd. Under the study conditions, the  48 h EC50 was determined to be 46 mg/L (van Wijk, 1994).

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989-03-05 to 1989-04-13

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Note, no analytical monitoring of the test substance concentration was performed.

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

A stock solution of 25 mg ethylene diamine/L was prepared in de-mineralised water. pH was neutralised to 7.0-7.3 using concentrated HCl. Using non neutralised solutions caused a change in pH outside of the range that could be tolerated by the test organisms.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Produced in house according to NEN 6503
- Age at study initiation: < 24 h

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

13.7°dH, 64.4 mg Ca/L, 20.7 mg Mg/L

Test temperature:

20.0-20.2 C

pH:

pH 7 - 7.5

Dissolved oxygen:

8.8-9 mg/L

Nominal and measured concentrations:

0, 5.6, 10, 32, 56 and 100 mg/L nominal concentrations

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker, 400 mL containing 250 mL test medium
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural water from turtle aquarium, used for culture

OTHER TEST CONDITIONS
- Adjustment of pH: Yes, using HCl
- Photoperiod: 16 h

EFFECT PARAMETERS MEASURED : Immobilisation

TEST CONCENTRATIONS
Range finding study
- Test concentrations: 0- 10 - 100 - 1000 mg/L

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

16.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 13.7 - 20.3mg/l

Reported statistics and error estimates:

95% confidence limits: 13.7 and 20.3 mg/L

In a preliminary test no effect was observed at the highest concentration used (32 mg/L). However the result of the second test is in line with the range finding test, and previous results obtained in the laboratory, and reported in literature.

Validity criteria fulfilled:

yes

Conclusions:

The 48 h EC50 to Daphnia magna is 16.7 mg/L.

Executive summary:

A study was conducted to determine the acute toxicity of the test substance to Daphnia magna, according to EU Method C.2, in compliance with GLP. The test concentrations were presented as nominal concentrations of 0, 5.6, 10, 32, 56 and 100 mg/L during the 48 h exposure. No analytical monitoring of test substance concentrations were performed in this study. The study was performed in 4 vessels per concentration, each containing 5 organisms. Under the study conditions, the 48 h EC50 was determined to be 16.7 mg/L (Balk, 1989).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

16.7 mg/L

Additional information

A study was conducted to determine the acute toxicity of the test substance to Daphnia magna according to EU Method C.2, in compliance with GLP. The test concentrations were presented as nominal concentrations of 0, 5.6, 10, 32, 56 and 100 mg/L during a 48 h exposure period. No analytical monitoring of test substance concentrations was performed. The study was carried out in 4 vessels per concentration, each containing 5 organisms. Under the study conditions, the 48 h EC50 was determined to be 16.7 mg/L (Balk, 1989).

A study was conducted to determine the acute toxicity of the test substance to Daphnia magna according to EU Method C.2 Guideline. Details on test substance concentrations, analytical monitoring and method used were not reported. Also, limited details were available on the results. Therefore adequate evaluation of the study results could not be performed. Under the study conditions, the  48 h EC50 was 46 mg/L (van Wijk, 1994).