Ethylenediamine

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Deviations:

yes

Remarks:

minor differnce in medium composition

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Strain ATCC 2262

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

22°C

Nominal and measured concentrations:

Three replicate sat each concentration, but no data on concentration interval.

Details on test conditions:

The culture medium was modified in that the concentration of KH2PO4 was increased from 1.6 to 160 mg/L and for NaHCO3 from 50 to 100 mg/L to improve buffer capacity of the medium.

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

151 mg/L

Basis for effect:

growth rate

Remarks on result:

other: standard deviation of mean 21.4

Validity criteria fulfilled:

not specified

Conclusions:

96 h EC50 for inhibition of algae growth rate was 150 mg/L.

Executive summary:

A study was conducted to determine the toxicity potential of the test substance to Selenastrum capricornutum, according to EU Method C.3. The growth inhibition of S. capricornutum has been assessed over an exposure period of 96 hours. No details of methods used and analytical monitoring were reported in the study. Under the study conditions, the 96 h EC50 was determined to be 150 mg/L (van Wijk, 1993).

Reference 2

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Note, no analytical monitoring of the test substance concentrations were performed.

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Version / remarks:

including updates published 1988

Deviations:

yes

Remarks:

Slight change in mineral medium, preliminary study performed but not included in report

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on test solutions:

A stock solution of 10 g Ethylene diamine /L in deionized water was titrated to neutral pH. Test solutions were prepared by dilution with test medium.

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Selenastrum capricornutum ATCC 22662 was mantained on mineral salts agar slants in the light at room temperature.

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Test temperature:

22 - 24°C

pH:

pH 7 in fresh solutions, pH in 72 h test medium was 7.5 - 9.

Nominal and measured concentrations:

0, 3.2, 10.2, 32.8, 104.9 and 335.5 mg/L nominal concentrations

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL Erlenmeyer flask containing 40 mL medium.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6


GROWTH MEDIUM
- Standard medium used: yes, with an increase of KH2PO4 and NaCO3 to 160 mg/L and 100 mg/L respectively to maintain a constant pH

OTHER TEST CONDITIONS
- Adjustment of pH: Yes, in stock solutions
- Photoperiod: Continuous
- Light intensity and quality: 6000 - 10000 lux

EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: spectrophotometer readings at 24 h intervals

TEST CONCENTRATIONS
- Range finding study was performed but not reported.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

71 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

645 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

- Exponential growth in the control : yes
- Observation of abnormalities: None

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- ErC50: 2.2 mg/L EbC50: 0.9 mg/L

Validity criteria fulfilled:

yes

Conclusions:

The 72 h ErC50 for algae was 645 mg/L and the 72 h NOEC was 3.2 mg/L.

Executive summary:

A static study was conducted to determine the toxicity of the test substance to Pseudokirchneriella subcapitata, according to EU Method C.3, in compliance with GLP. The exponentially growing P. subcapitata has been assessed over an exposure period of 72 h. In the definitive test the algae were exposed to the nominal concentrations of 0, 3.2, 10.2, 32.8, 104.9 and 335.5 mg/L. No analytical monitoring of the test substance concentrations were performed. Under the study conditions, the 72 h EbC50 and ErC50 of the test substance were determined to be 71 and 645 mg/L, respectively. The 72 h NOEC was determined to be 3.2 mg/L (van Ginkel, 1990).

Reference 3

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Well documented study performed according to Industrial standard

Qualifier:

according to guideline

Guideline:

other: DIN 98 412, Part 9, 1988

GLP compliance:

not specified

Analytical monitoring:

no

Details on test solutions:

Stock solution prepared in double-distilled water. pH adjusted to 7. 7- 8.3

Test organisms (species):

Scenedesmus capricornutum

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test temperature:

23- 25°C

Nominal and measured concentrations:

Dilution steps 1:2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Basis for effect:

growth rate

Validity criteria fulfilled:

not specified

Conclusions:

The 48 h EC50 to Scenedesmus capricornutum was > 100 mg/L.

Executive summary:

A static study was conducted to determine the toxicity of the test substance to Scenedesmus capricornutum according to DIN 98 412 (Part 9, 1988). The growth inhibition of S. capricornutum was assessed over an exposure period of 48 h. Details of method and analytical monitoring were not reported. Under the study conditions, the 48 h EC50 was determined to be > 100 mg/L (Kuhn, 1990).

Key value for chemical safety assessment

EC50 for freshwater algae:

645 mg/L

EC10 or NOEC for freshwater algae:

3.2 mg/L

Additional information

A static study was conducted to determine the toxicity of the test substance to Pseudokirchneriella subcapitata according to EU Method C.3, in compliance with GLP. The exponentially growing P. subcapitata were assessed over an exposure period of 72 h. In the definitive test, the algae were exposed to nominal concentrations of 0, 3.2, 10.2, 32.8, 104.9, and 335.5 mg/L. No analytical monitoring of the test substance concentrations was performed. Under the study conditions, the 72 h EbC50 and ErC50 of the test substance were determined to be 71 and 645 mg/L, respectively. The 72 h NOEC was 3.2 mg/L (van Ginkel, 1990).

A static study was conducted to determine the toxicity of the test substance to Scenedesmus capricornutum according to DIN 98 412 (Part 9, 1988). The growth inhibition of S. capricornutum was assessed over an exposure period of 48 h. Details of method and analytical monitoring were not reported. Under the study conditions, the 48 h EC50 was determined to be > 100 mg/L (Kuhn, 1990).

A study was conducted to determine the toxicity potential of the test substance to Selenastrum capricornutum according to EU Method C.3. The growth inhibition of S. capricornutum was assessed over an exposure period of 96 h. No details of methods used and analytical monitoring were reported. Under the study conditions, the 96 h EC50 was determined to be 150 mg/L (van Wijk, 1993).