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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable study which meets basic scientific principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1952

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
14 day mortality of rats exposed to metered concentrations of EDA vapor for 8h.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethylenediamine
EC Number:
203-468-6
EC Name:
Ethylenediamine
Cas Number:
107-15-3
Molecular formula:
C2H8N2
IUPAC Name:
ethane-1,2-diamine

Test animals

Species:
rat
Strain:
not specified
Sex:
male

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
8 h
Concentrations:
2000 ppm corresponding to 4.9 mg/l and 4000 ppm corresponding to 9.8 mg/l
No. of animals per sex per dose:
6 animals per dose
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LC0
Effect level:
4.9 mg/L air
Exp. duration:
8 h
Sex:
male
Dose descriptor:
LC100
Effect level:
9.8 mg/L air
Exp. duration:
8 h
Sex:
male
Dose descriptor:
LC50
Effect level:
7.35 mg/L air
Exp. duration:
8 h
Remarks on result:
other: Geometric mean of LC0 and LC100
Sex:
male
Dose descriptor:
LC50
Effect level:
14.7 mg/L air
Exp. duration:
4 h
Remarks on result:
other: Back calculated from 8h exposure using Habers law

Applicant's summary and conclusion

Interpretation of results:
Toxicity Category IV
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Acute inhalation LC50 was 14.7 mg/l.
Executive summary:

Rats were exposed to metered concentrations of EDA vapor for 8h. Six male rats per dose group were exposed to 2000 and 4000 ppm EDA vapour for 8h. Mortality of rats was observed for a 14 day period. No deaths were observed in the 2000 ppm dose group, and 100% mortality was observed in the 4000 ppm dose group. Taking the geometric mean as the LC50 value, and calculating back to 4h exposure gives an LC50 of 14.7 mg/l