Sodium 3-nitrobenzenesulphonate

Endpoint summary

Description of key information

Bioaccumulation: aquatic / sediment

Bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical.The study was performed according to OECD Guideline 305 C "Bioaccumulation: Test for the Degree of Bioconcentration in Fish" andother guideline"Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively under flow through conditions at a temperature of25°C and pH range 6.0-8.5, respectively.Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 1 mg/l and 0.1 mg/l, respectively. Range finding study involve theTLm(48h) > 1000 mg/LonRice fish(Oryzias latipes).The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be in the range of 0.5-1.6 L/Kg at a conc. of 1 mg/l and ≤ 5 L/Kg at a conc. of 0.1 mg/l, respectively, which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.

Additional information

Bioaccumulation: aquatic / sediment:

Experimental study and predicted data for the target chemical and various supporting studies for its structurally similar read across substances were reviewed for the bioaccumulation end points which are summarized as below:

 In an experimental key study from authoritative database (J-CHECK & BCF database, 2017 and EnviChem, 2014) and secondary source (IUCLID dataset, 2000) for the target chemical, bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical .The study was performed according to OECD Guideline 305 C "Bioaccumulation: Test for the Degree of Bioconcentration in Fish" and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively under flow through conditions at a temperature of25°C and pH range 6.0-8.5, respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 1 mg/land 0.1 mg/l. Range finding study involve the TLm(48h) > 1000 mg/L on Rice fish(Oryzias latipes).The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be in the range of 0.5-1.6 L/Kg at a conc. of 1 mg/l and ≤ 5 L/Kg at a conc. of 0.1 mg/l, respectively.

From CompTox Chemistry Dashboard using OPERA (OPEn (quantitative) structure-activity Relationship Application)  V1.02 model in which calculation based on PaDEL descriptors (calculate molecular descriptors and fingerprints of chemical), the bioaccumulation i.e BCF for test chemical was estimated to be 5.05 dimensionless . The predicted BCF result was based on the 5 OECD principles.

In a next weight of evidence study from authoritative database (J-CHECK, 2017) for the test chemical, bioaccumulation experiment was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical. The study was performed according to OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish) and other guideline "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", respectively. Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 0.5mg/land 0.05 mg/l, respectively. Analytical method involve the recovery ratio: Test water: Fish: 91.0 %, - Limit of quantitation: Test water: 1st concentration area : 0.018 mg/L, 2nd concentration area : 0.0018 mg/L, Fish : 0.20 microg/g. Range finding study involve theLC50 (48h) 326 mg/L on Rice fish (Oryzias latipes). Lipid content of the test organism Cyprinus carpio was determined to be 4.1% at the start of exposure. The bioconcentration factor (BCF value) of test chemical on Cyprinus carpio was determined to be ≤ 0.4 L/Kg at a conc. of 0.5 mg/l and ≤ 4.1 L/Kg at a conc. of 0.05 mg/l, respectively.

In another weight of evidence study for test chemical, the bioaccumulation study was conducted on test organism Cyprinus carpio for 6 weeks for evaluating the bioconcentration factor (BCF value) of test chemical (J-CHECK, 2017). Cyprinus carpio was used as a test organism for the study. Test chemical nominal conc. used for the study were 1mg/land 0.1 mg/l, respectively. Range finding study involves the TLm (48 hr) ˃ 1000 mg/l (w/v) on Rice fish (Oryzias latipes). The bioconcentration factor (BCF value) test chemical on Cyprinus carpio was determined to be in the range of 0.2-0.5 L/Kg at a conc. of 1 mg/l and ≤ 2 L/Kg at a conc. of 0.1 mg/l, respectively.

On the basis of above results for target chemical (from authoritative J-CHECK, BCF database, EnviChem, secondary source & modelling database CompTox Chemistry Dashboard,2017) it can be concluded that the BCF value of test chemical ranges from 0.5–5.05 which does not exceed the bioconcentration threshold of 2000, indicating that the test chemical is not expected to bioaccumulate in the food chain.