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Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
Japanese government peer-reviewed the documents, audited selected studies.
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
no
Specific details on test material used for the study:
Supplier: KISHIDA CHEMICALS CO., Ltd (Lot No. D12358J)
Purity: >= 98 %
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Test Organism:
-strain: ATCC-22662
-supplier: American Type Culture Collection
-acclimation: 3 days
-initial cell concentration: 1X10E+4 cells/mL
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Test temperature:
original test: 21.8 - 22.8 (mean 22.5) °C
supplemental test: 21.2 - 23.0 (mean 22.5) °C
pH:
original test: 7.0 - 7.3 at start, 8.1-8.6 at end of test
supplemental test: 7.3 - 7.5 at start, 8.9 - 9.0 at end of test
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
0, 1000 mg/L (original test, nominal) actual concentration measured and greater than 80% of the nominal concentration
0, 556 mg/L (supplemental test, nominal) actual concentration measured and greater than 80% of the nominal concentration
Details on test conditions:
TEST MEDIUM CHEMISTRY: OECD medium
TEST SYSTEM (closed system)
-exposure vessel type: 100 mL medium in a 300 mL Erlenmeyer flask with a porous plug
-number of replicates: 3
-test temperature: 21 - 25 degree C
-pH: no adjustment during exposure period
-intensity of irradiation: 4,000 - 5,000 lux
-photoperiod: continuous
-shaking: 100 rpm
TEST PARAMETER: cell concentration (cells/mL)
Reference substance (positive control):
yes
Remarks:
K2Cr2O7
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: actual concentration measured and greater than 80% of the nominal concentration
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: actual concentration measured and greater than 80% of the nominal concentration
Details on results:
Results:
Table 1 NOMINAL/MEASURED CONCENTRATION
-----------------------------------------------------------
Nominal Measured conc. (mg/L) conc.
(% of nominal) (mg/L) 0 hr* 72 hr**
-----------------------------------------------------------
Control <0.5 <0.5
1000 mg/L 1011 (101.1) 926 (92.6)

Control <0.5 <0.5
556 mg/L 599 (107.7) 556 (100.0)
-----------------------------------------------------------
*: initial **: final


EFFECT DATA/ELEMENT VALUES:
-area method: EbC50 (0 - 72hr ) > 1000 mg/L
NOEbC (0 - 72hr ) = 556 mg/L
-rate method: ErC50 ( 24 - 48hr ) > 1000 mg/L
NOErC ( 24 - 48hr ) = 1000 mg/L
ErC50 ( 24 - 72hr ) > 1000 mg/L
NOErC ( 24 - 72hr ) = 1000 mg/L

Table 2 AVERAGE CELL DENSITY OF Selenastrum capricornutum
-----------------------------------------------------------
Nominal conc. Cell Density (1x10E+4 cell/mL)
(mg/L) 0 hr 24 hr 48 hr 72 hr
-----------------------------------------------------------

Control 1.0 5.5 34.7 201.6
1000mg/L 1.0 5.0 30.6 197.3

Control 1.0 5.2 33.5 204.4
556mg/L 1.0 5.3 40.6 212.2
-----------------------------------------------------------
Table 3 AVERAGE GROWTH INHIBITION
-----------------------------------------------------------
Nominal Inhibition
conc. area method growth rate growth rate
(mg/L) (0-72hr)% (24-48hr)% (24-72hr)%
-----------------------------------------------------------

Control - - -
1000 mg/L 4.82 1.59 -2.08

Control - - -
556 mg/L -7.99 -9.36 -0.34
-----------------------------------------------------------
Results with reference substance (positive control):
REFERENCE SUBSTANCE: K2Cr2O7 EbC50 (72hrs) = 0.52 mg/L
Reported statistics and error estimates:
STATISTICS: F&T-test; Yukms StatLight #3 "Pairwise Comparisons" EA Japan
Validity criteria fulfilled:
yes
Conclusions:
At a limit concentration of 1000 mg/l (nomial), methacrylamide showed no growth inhibition in an Algae, Growth Inhibition Test OECD 201.
ErC50 (72h) > 1000 mg/l (measured/nominal), NOErC = 1000 mg/l (measured/nominal)
Executive summary:

In a 72-hour acute toxicity study, the cultures of Pseudokirchneriella subcapitata, strain ATCC-22662 were exposed to control and test item at nominal/measured concentrations of 1000 mg a.i./L in accordance with the Algae, Growth Inhibition Test, OECD 201  The NOEC and ErC50values based on growth inhibition were 1000 mg/l and > 1000 mg a.i./L, respectively.  The % growth inhibition in the treated algal culture as compared to the control ranged from -2.08 to 1.59 

There were no compound related phytotoxic effects.

This toxicity study is classified as acceptable and satisfies the guideline requirements for Algae, Growth Inhibition study.

Results Synopsis:

Test organism: Pseudokirchneriella subcapitata

Test type: static

72 -hr EC50: > 1000 mg/l

72 -hr NOEC: 1000 mg/l

Description of key information

In a 72-hour acute toxicity study, the cultures of Pseudokirchneriella subcapitata, strain ATCC-22662 were exposed to control and test item at nominal/measured concentrations of 1000 mg a.i./L in accordance with the Algae, Growth Inhibition Test, OECD 201  The NOEC and ErC50values based on growth inhibition were 1000 mg/l and > 1000 mg a.i./L, respectively.  The % growth inhibition in the treated algal culture as compared to the control ranged from -2.08 to 1.59 

There were no compound related phytotoxic effects.

This toxicity study is classified as acceptable and satisfies the guideline requirements for Algae, Growth Inhibition study.

Key value for chemical safety assessment

EC50 for freshwater algae:
1 000 mg/L
EC10 or NOEC for freshwater algae:
1 000 mg/L

Additional information