Registration Dossier

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2008

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Version / remarks:
2001
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
Supplier: Merck
Purity: > 98 %

Test solutions

Vehicle:
no
Details on test solutions:
The test vessel contains
- 10 ml of synthetic medium (peptone, meat extract, urea, sodium chloride, calcium chloride, magnesium sulphate, potassium monohydrogen phosphate
- 2-4 g d.w.w/l of activated sludge in stirring condition with a magnetic stirrer

Test organisms

Test organisms (species):
activated sludge
Details on inoculum:
no data

Study design

Test type:
static
Water media type:
freshwater

Test conditions

Test temperature:
22 °C +/- 2 °C
pH:
7.5 +/- 0.5
Dissolved oxygen:
-initial oxygene concentration: close to saturation limit
Nominal and measured concentrations:
-Test substance concentration: 10-30 g/l methacrylamide

Results and discussion

Effect concentrations
Dose descriptor:
EC50
Effect conc.:
24 800 mg/L
Basis for effect:
inhibition of total respiration
Remarks on result:
other:
Details on results:
Calculated inhibition range: 22-58 %

Applicant's summary and conclusion

Executive summary:

Methacrylamide was tested in an activated sludge inhibition test acc. ISO 8192. Methacrylamide was tesed in concetration of 10 -30 g/l. EC50 activated sludge inhibition was determined with 24800 mg/l