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Toxicological information

Neurotoxicity

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Administrative data

Endpoint:
neurotoxicity
Remarks:
subacute
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented study which meets basic scientific principles.

Data source

Reference
Reference Type:
publication
Title:
Effects of acrylamide and acrylic acid on creatine kinase activity in the rat brain.
Author:
Kohriyama, K. et al.
Year:
1994
Bibliographic source:
Arch. Toxicol., 68, 67-70

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Objective: investigation into the biochemical mechanisms of neurotoxicity of acrylamide. Acrylic acid (AA) was examined as an analogue for comparison.
Creatinkinase activity (CK) -considered as a key enzyme in brain metabolism- served as putative indicator for cerebral impairment (correlation between inhibition of CK and regional vulnerability in brain?).
GLP compliance:
not specified
Limit test:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
no data

Test animals

Species:
rat
Strain:
Wistar
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: 0.85% NaCI
Analytical verification of doses or concentrations:
yes
Duration of treatment / exposure:
8 d
Frequency of treatment:
on consecutive days
Doses / concentrations
Remarks:
Doses / Concentrations:
50 mg/kg bw
Basis:
analytical conc.
No. of animals per sex per dose:
7
Control animals:
yes, concurrent vehicle
Details on study design:
Studies were performed in male Wistar rats (7 per group) in vitro and in vivo. In-vivo doses were 8x 50 mg/kg bw administered ip. on consecutive days.
Distribution of test material in the brain was checked separately, using 1-14C-labelled substance. The activity of certain enzymes (i.e. CK, LDH, ASAT) was determined

Results and discussion

Any other information on results incl. tables

Clinical observation: Rats treated with AA did not exhibit any impairment of movement, contrary to those treated with acrylamide (splay of hind limbs and awkward gait), but body weight gain was significantly reduced (> 20 %).
Enzyme activities: In vitro AA inhibited CK in dose-related manner, distinctly more pronounced than acrylamide. In vivo, no such inhibition of CK was observed following AA-treatment, but was seen with acrylamide.

This finding seems to correspond to the distinctly higher retention of radioactivity found after ip. infusion of labelled acrylamide than of labelled AA.

Applicant's summary and conclusion