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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1964
Report date:
1964

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
no
Principles of method if other than guideline:
Method: BASF-Test
GLP compliance:
no
Test type:
other: Inhalation hazard test
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other:

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
not specified
Vehicle:
air
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
8 h
Concentrations:
0.69 mg/L
No. of animals per sex per dose:
6
Control animals:
not specified
Details on study design:
This test (also called inhalation risk test) was performed in principle as described in OECD Guideline 403. It demonstrates the toxicity of an atmosphere saturated with vapors of the volatile components of a test substance at the temperature chosen for vapor generation (here 20 °C). Groups of 3 rats per sex were exposed to the vapors, generated by bubbling 200 L/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 8 hours. For confirmation of the results, the experiment was repeated once. No analytical determination of the atmosphere concentrations was performed.
The nominal concentration was calculated as quotient of the amount of test substance weight loss during the exposure and the amount of air used during the exposure.

Group-wise documentation of clinical signs was performed over the 7-day study period. Body weight of groups was determined before the start of
the study and at the end of the observation period in the surviving animals. The clinical signs and findings were reported in summarized form. Upon completion of the study, all animals were sacrificed and submitted to gross-pathological examination. The study allows for an estimate of the length of time required to cause severe toxic effects resulting from exposure to an atmosphere saturated with volatile components of the test substance.

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 0.69 mg/L air
Based on:
test mat.
Exp. duration:
8 h
Remarks on result:
other: no mortality occured
Mortality:
No mortality occurred within 8 hours and during the post-exposure period of 7 days after inhalation of a highly saturated vapor-air mixture of the test compound.
Clinical signs:
other: No clinical signs or symptoms were noted.
Gross pathology:
No macroscopic pathologic abnormalities were noted in the surviving animals examined at the end of the observation period.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met