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EC number: 205-571-1 | CAS number: 142-91-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 1981-09-09 to 1991-09-11
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- limited documentation, incomplete strain selection
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Isopropyl myristate
- EC Number:
- 203-751-4
- EC Name:
- Isopropyl myristate
- Cas Number:
- 110-27-0
- Molecular formula:
- C17H34O2
- IUPAC Name:
- isopropyl myristate
- Details on test material:
- - Name of test material (as cited in study report): Isopropylmyristate
- Analytical purity: no data
Constituent 1
Method
- Target gene:
- Genes involved in Histidine synthesis
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other:
- Species / strain / cell type:
- S. typhimurium TA 1538
- Additional strain / cell type characteristics:
- other:
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-Mix (Aroclor 1254 induced)
- Test concentrations with justification for top dose:
- 0, 4, 20, 100, 500 and 2500 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Acetone
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- water
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-Nitro-o-phenylendiamin
- Remarks:
- TA1537, TA1538 and TA98 without metabolic activation
- Untreated negative controls:
- yes
- Remarks:
- water
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- TA1535 and TA100 without metabolic activation
- Untreated negative controls:
- yes
- Remarks:
- water
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Remarks:
- All strains with metabolic activation
- Evaluation criteria:
- Revertant colonies were counted and the means and standard deviations were calculated and compared to the controls.
- Statistics:
- Means and standard deviations were calculated
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- ADDITIONAL INFORMATION ON CYTOTOXICITY:
The vehicle acetone with 100 µL/plate had cytotoxic effects on the tester strains TA 100 and TA 1537 with and without metabolic activation. Therefore additional experiments with 50µL acetone/plate with these two strains were performed. These experiments lead to valid results except for TA 1537 without activation. To obtain reliable results and for the comparability of the two series of experiments, the relative numbers of revertants in comparison to the vehicle control were calculated (Table 2).
Any other information on results incl. tables
Table 1: Maximum number of revertants
|
Maximum number of revertants (dose level [µg/mL]) |
|||||
Negative control |
Positive control |
Treatment |
||||
Strain |
With S9 |
Without S9 |
With S9 |
Without S9 |
With S9 |
Without S9 |
TA 98 |
24 |
20 |
796 |
843 |
25 (2500) |
18 (4) |
TA 100 |
150 |
49 |
935 |
375 |
128 (500) |
49 (500) |
TA 1535 |
13 |
6 |
145 |
336 |
11 (2500) |
9 (2500) |
TA 1537 |
13 |
8 |
275 |
-- |
10 (4) |
-- |
TA 1538 |
27 |
16 |
376 |
662 |
20 (100) |
17 (500) |
Table 2: Maximum relative number of revertants
|
Maximum relative number of revertants (dose level [µg/mL]) |
|||||
Negative control |
Positive control |
Treatment |
||||
Strain |
With S9 |
Without S9 |
With S9 |
Without S9 |
With S9 |
Without S9 |
TA 98 |
1 |
1 |
33.6 |
42.2 |
1.03 (2500) |
0.90 (4) |
TA 100 |
1 |
1 |
6.2 |
7.6 |
0.85 (500) |
0.99 (500) |
TA 1535 |
1 |
1 |
11.2 |
58.9 |
0.81 (2500) |
1.58 (2500) |
TA 1537 |
1 |
1 |
21.5 |
-- |
0.74 (4) |
-- |
TA 1538 |
1 |
1 |
13.9 |
40.6 |
0.72 (100) |
1.01 (500) |
The results for TA 1537 without S9 -mix were not valid. Although the targets of reverse mutations are different in TA 1537 and TA 98, the possible mutational event in both strains is frameshift. TA 98 is additionally more sensitive to mutations because it carries the R-factor (pKM101) plasmid. The fact that there is no increase in revertants in TA 98 without S9 -mix, gives evidence that there would be no increase of revertants in TA 1537 as well. Therefore it can be concluded that the test substance showed no increase in the number of revertants at any concentration in any tester strain. The test substance is non-mutagenic.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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