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Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 Sept 1996 to 13 Nov 1996
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted in accordance with relevant OECD guideline and to GLP standards.
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
yes
Specific details on test material used for the study:
- Name of test material (as cited in study report): AMYL SALICYLATE
- Molecular formula (if other than submission substance): C12H16O3
- Molecular weight (if other than submission substance):208.3 g/mol
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: Mixture of two isomers (ratio not reported)
- Physical state: Liquid
- Analytical purity: 98.5% (sum of two isomers)
- Lot/batch No.: 261891
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Duration of test (contact time):
28 d
Initial conc.:
100.4 mg/L
Based on:
test mat.
Initial conc.:
101.5 mg/L
Based on:
test mat.
Initial conc.:
102.6 mg/L
Based on:
test mat.
Initial conc.:
101.7 mg/L
Based on:
test mat.
Parameter:
% degradation (O2 consumption)
Remarks:
BOD, mgO2/L adjusted to nominal concentrations
Value:
32
Sampling time:
2 d
Remarks on result:
other: Mean of two tests
Parameter:
% degradation (O2 consumption)
Remarks:
BOD, mgO2/L adjusted to nominal concentrations
Value:
63
Sampling time:
7 d
Remarks on result:
other: Mean of two tests
Parameter:
% degradation (O2 consumption)
Remarks:
BOD, mgO2/L adjusted to nominal concentrations
Value:
80
Sampling time:
12 d
Remarks on result:
other: Mean of two tests
Parameter:
% degradation (O2 consumption)
Remarks:
BOD, mgO2/L adjusted to nominal concentrations
Value:
83
Sampling time:
14 d
Remarks on result:
other: Mean of two tests
Parameter:
% degradation (O2 consumption)
Remarks:
BOD, mgO2/L adjusted to nominal concentrations
Value:
86
Sampling time:
28 d
Remarks on result:
other: Mean of two tests

Reference material: Aniline (99.5% min.)


 


Toxicity control:


The toxicity of the test substance to the innoculum was checked by measuring the inhibitory rate on respiration compared to a control sample with test substance. The toxicity control and control contained the following:


Toxicity control: Mineral medium + test substance (100mg/L) + aniline (100mg/L, nominal) + innoculum


Control: Mineral medium + aniline (100mg/L, nominal) + innoculum.


 


Toxicity control attained 81% degradation after 28 -days, which demonstrates the non-inhibitory effect of the test substances on the inoculum used in the test.


 


Control: In the flasks containing aniline alone, pH remained roughly at the same level, which suggests that the final form of nitrogen is partly one of the acid forms NO2 - or NO3- and partlly the alkaline ion NH4 +. The ThOD corresponds to NO3- and was used for the calculation of the biodegradation rates of aniline. Using the ThOD corresponding to NH4+ would lead to higher degradation rates. In both cases, degradation of aniline exceeds 40% after 7 days and 65% after 14 days. The activity of the innoculum is thus verified and the test is considered as valid.

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
The test substance underwent 86% biodegradation after 28 days in the test conditions. Biodegradation started on day 2 and reached 80% at the end of the 10-day window. The substance is therefore considered to be readily biodegradable under the conditions of the test.
Executive summary:

The ready biodegradation potential of the test substance, Amyl salicylate, was determined using the OECD guideline 301F (Manometric respirometry test) over a test period of 28-days.


Amyl salicylate attained 86% biodegradation after 28-days under the conditions of the test. Biodegradation started on day 2 and reached 80% at the end of the 10 -day window. Thus, Amyl salicylate is considered to be readily biodegradable.


The substance was exposed to innoculum, prepared from predominantly domestic sewage, at nominal concentration of 100mg/L at approximately 22 degC.


A further similar test sample was used as a toxicity control, which attained greater than 80% degradation after 28-days, thus confirming the non-inhibitory effect of the test substance on the innoculum. A control sample of mineral medium, innoculum and aniline confirmed the actvity of the innoculum used.

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions. A reference substance was not included in the study but the high level of degradation seen for the test item confirms that the inoculum was viable)
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
yes
Remarks:
Modified by using sealed test vessels to allow assessment of volatile substances.
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
- Name of test material (as cited in study report): Amyl salicylate
- Molecular weight (if other than submission substance): 208.3
- Structural formula attached as image file (if other than submission substance): see Fig.
- Physical state: liquid
- Analytical purity: 100% nominal
- Lot/batch No.: R456
- Storage condition of test material: Store at room temperature in the dark.
- Other: Percentage Carbon = 69.1%
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial, non-adapted
Duration of test (contact time):
28 d
Initial conc.:
10 mg/L
Based on:
other: Organic carbon
Parameter followed for biodegradation estimation:
CO2 evolution
Parameter:
% degradation (CO2 evolution)
Value:
27.2
Sampling time:
4 d
Parameter:
% degradation (CO2 evolution)
Value:
66.9
Sampling time:
14 d
Parameter:
% degradation (CO2 evolution)
Value:
81.3
Sampling time:
28 d
Conclusions:
The substance is considered to be readily biodegradable after attaining 81.3% degradation within 28-days in a CO2 evolution ready biodegradation study and passing the 10 day window criterion.
Executive summary:

The substance is considered to be readily biodegradable after attaining 81.3% degradation within 28-days in a CO2 evolution ready biodegradation study and passing the 10 -day window criterion.

Description of key information

Readily biodegradable. The test substance exceeded the pass level of 60% within 28 days in two ready biodegradability tests. The  10-day window criterion was met in both studies. 

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable
Type of water:
freshwater

Additional information

The ready biodegradation potential of the test substance, Amyl salicylate, was determined using the OECD guideline 301F over a test period of 28-days (key study). Amyl salicylate attained 86% biodegradation after 28-days under the conditions of the test. Biodegradation started on day 2 and reached 80% at the end of the 10-day window. Thus, Amyl salicylate is considered to be readily biodegradable.

The substance was exposed to innoculum, prepared from predominantly domestic sewage, at nominal concentration of 100mg/L at approximately 22 degC. A further similar test sample was used as a toxicity control, which attained greater than 80% degradation after 28-days, thus confirming the non-inhibitory effect of the test substance on the innoculum.

In a supporting CO2 evolution ready biodegradation study, the substance attained 81.3% degradation within 28-days in a CO2 evolution ready biodegradation study. The 10 day window criterion was also met.