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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study OECD 203, C.1 resp.
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
92/69/EEC
Deviations:
no
GLP compliance:
yes (incl. certificate)
Specific details on test material used for the study:
- Name of test material (as cited in study report): AMYL SALICYLATE
- Structural formula attached as image file (if other than submission substance): see Fig.





Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
96 h
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
0.95 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: arithm. mean of fresh and 24h old test solution
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
1.9 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: arithm. mean of fresh and 24h old test solution
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.34 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: LC50 is geometric mean of LC0 and LC100
Validity criteria fulfilled:
yes
Conclusions:
Following exposure for 96 hours to groups of 10 fish at nominal concentrations of 0.8mg/L, 1.1mg/L, 1.6mg/L, 2.3mg/L 3.3mg/L, 4.7mg/L and a control containing synthetic water an arithmetic mean measured LC0 was determined to be 0.95mgL and the LC100 was determined to be 1.9mg/L.
Executive summary:

The acute toxicity to Zebra fish was determined following the test guideline C.1 in accordance with Council Directive 92/69/EEC. Following exposure for 96 hours to groups of 10 fish at nominal concentrations of 0.8mg/L, 1.1mg/L, 1.6mg/L, 2.3mg/L 3.3mg/L, 4.7mg/L and a control containing synthetic water, an arithmetic mean measured LC0 was determined to be 0.95mgL and the LC100 was determined to be 1.9mg/L. The LC50 was determined to be 1.34mg/L (geometric mean of LC0 and LC100).

Description of key information

LC50(96hrs)= 1.34 mg/L

Key value for chemical safety assessment

LC50 for freshwater fish:
1.34 mg/L

Additional information

The acute toxicity to Zebra fish was determined in a semi-static test design with daily renewal of the test media. Following exposure for 96 hours to groups of 10 fish at nominal concentrations of 0.8mg/L, 1.1mg/L, 1.6mg/L, 2.3mg/L 3.3mg/L, 4.7mg/L and a control containing synthetic water, no mortality was observed at the nominal concentration of 3.3mg/L while 100% mortality was observed at 4.7mg/L. Analysis was performed on newly prepared media and media after 24 hours exposure. The arithmetic mean measured concentrations corresponding to the LC0 and LC100 were 0.95 and 1.9 mg/L respectively. The LC50 was determined to be 1.34mg/L (geometric mean of LC0 and LC100).