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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Documentation insufficient for assessment. Only secondary literature. Original report not available.

Data source

Referenceopen allclose all

Reference Type:
secondary source
Title:
Unnamed
Year:
1999
Reference Type:
publication
Title:
Studies on the toxicity of pulp and paper mill effluents—I. Mutagenicity of the sediment samples derived from Kraft paper mills
Author:
Kinae N. et al.
Year:
1979
Bibliographic source:
Water Research, Vol. 15, p17, 1979

Materials and methods

Principles of method if other than guideline:
Ames test (TA 98, TA 100, TA 1537) and bacillus subtillis assay
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl palmitate
EC Number:
203-966-3
EC Name:
Methyl palmitate
Cas Number:
112-39-0
Molecular formula:
C17H34O2
IUPAC Name:
methyl palmitate
Details on test material:
- Name of test material (as cited in study report): methyl palmitate as component of sediment samples collected below a paper mill, compound was isolated and tested
- Analytical purity: not given

Method

Target gene:
his operon
Species / strain
Species / strain / cell type:
other: TA 98, TA 100, TA 1537 & Bacillus subtillis
Additional strain / cell type characteristics:
not specified
Metabolic activation:
not specified
Test concentrations with justification for top dose:
100 µg/plate
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified

Results and discussion

Any other information on results incl. tables

Ames test and Bacillus subtillis assay (rec-effect 10 mg/disk) were found to be negative.

Applicant's summary and conclusion