Chloroacetone

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1996-05-14 to 1996-08-23

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: According to DIN Guideline under GLP, dose range selection not comprehensive.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, Teil 15 Juni 1982

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Chloroacetone

EC Number:

201-161-1

EC Name:

Chloroacetone

Cas Number:

78-95-5

Molecular formula:

C3H5ClO

IUPAC Name:

1-chloropropan-2-one

Details on test material:

- Name of test material (as cited in study report): Monochloracetone
- Physical state: liquid
- Analytical purity: 96.18 %
- Impurities (identity and concentrations): 1,3-Dichloracetone (0.5%); 4-Methylpent-3-en-2-on (1%); 1,1-Dichloracetone (3%)
- Lot/batch No.: 603015
- Expiration date of the lot/batch: March 12, 1997
- Stability under test conditions: room temperature, dark
- Storage condition of test material: moderat stable

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methanol
- Stock solution: 200mg test substance + 5000 mg methanol in 1 L test water
- Renewal: After 24 hours

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name:
- Strain:
- Source: Gunter Rosenhainer, 06766 Thalheim
- Length at study initiation (length definition, mean, range and SD): 6.5 cm
- Weight at study initiation (mean and range, SD): 2.4 g
- Feeding during test
- Food type: frozen larve mosquitos
- Frequency: daily until 48 h before test start

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

-

Test conditions

Hardness:

c(Ca,Mg)= 2.5 mmol

Test temperature:

19.5 -21.0 °C

pH:

7.8-7.9

Dissolved oxygen:

9.0 at 0h

Salinity:

-

Nominal and measured concentrations:

nominal 0, 0.1, 0.2, 0.4, 0.8, 1.6

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.6 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

0.2 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Monochloracetone was very toxic to fish under the reported conditions of the test.

Executive summary:

The acute toxicity of monochloroacetone to fish (Leuciscus idus) was tested in a semi-static test according to DIN 38412, Teil 15 Juni 1982. The fish were exposed to the substance in a nominal concentration range from 0.1 mg/L to 1.6 mg/L for 48 hours. The measured concentrations did not drop below 80% of the nominal values. No mortalities occured during the test in the control. The 48 -hour LC50 was 0.6 mg/L.