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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Description of key information

Effects are most likely related to the light absorption caused by the blue color of the test substance at the highest test concentrations.

Key value for chemical safety assessment

Additional information

One study is available investigating the effects of Milori blue (CAS 25869-00-5) on the freshwater algae Selenastrum capricornutum (Saito, 1996). Since the test substance is insoluble in water (< 0.02 mg/L) the test solutions were prepared using a solvent to result in test solutions up to 16 mg/L (nominal). After a test duration of 72 h an EC50 of 9.7 mg/L and the NOEC of 8 mg/L based on the growth rate resulted. However, since the test substance is a pigment it has probably decreased the light transmission to the medium and suppressed the algal growth. Thus, the effects are most likely related to the blue colour of the test medium. Increasingly blue color was observed in the test solutions with increasing concentration of the test substance. Moreover, a slight amount of blue precipitate was observed at the highest concentration. Despite the insolubility of the substance without a vehicle is unlikely that the substance is acutely harmful to algae, the effects are related to the light-absorbing pigment. Therefore, the results are not relevant for the assessment.